Recombinant human PKN3 protein (ab125615)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 75% Densitometry
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: WB, Functional Studies, SDS-PAGE
Description
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Product name
Recombinant human PKN3 protein -
Biological activity
The specific activity of ab125615 was determined to be 3 nmol/mg/min.
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Purity
> 75 % Densitometry.
Affinity purified. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Predicted molecular weight
130 kDa including tags -
Amino acids
1 to 889 -
Tags
GST tag N-Terminus
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Specifications
Our Abpromise guarantee covers the use of ab125615 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
Functional Studies
SDS-PAGE
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Form
Liquid -
Additional notes
ab204879 (RPS6 peptide) can be utilized as a substrate for assessing kinase activity
This product was previously labelled as PKN beta
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol (glycerin, glycerine), 0.88% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- pkn3
- PKN3_HUMAN
- PKNBETA
see all -
Function
Contributes to invasiveness in malignant prostate cancer. -
Tissue specificity
Expressed in prostate tumors and various cancer cell lines. Not expressed in adult tissues. -
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 protein kinase domain.
Contains 3 REM (Hr1) repeats. -
Domain
The C1 domain does not bind the diacylglycerol (DAG). -
Post-translational
modificationsAutophosphorylated. -
Cellular localization
Nucleus. Cytoplasm > perinuclear region. Nuclear and perinuclear Golgi region. - Information by UniProt
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab125615 has not yet been referenced specifically in any publications.