Recombinant human PLK3 protein (ab125627)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 75% Densitometry
- Active: Yes
- Suitable for: WB, Functional Studies, SDS-PAGE
Description
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Product name
Recombinant human PLK3 protein
See all PLK3 proteins and peptides -
Biological activity
The specific activity of PLK3 was determined to be 2.2 nmol/min/mg.
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Purity
> 75 % Densitometry.
Affinity purified. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Predicted molecular weight
60 kDa including tags -
Amino acids
57 to 340
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Specifications
Our Abpromise guarantee covers the use of ab125627 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
Functional Studies
SDS-PAGE
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Form
Liquid -
Additional notes
ab91090 (Cow Casein full length protein) can be utilized as a substrate for assessing Kinase activity
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol (glycerin, glycerine), 0.88% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- CNK
- Cytokine Inducible Kinase
- Cytokine-inducible serine/threonine-protein kinase
see all -
Function
Serine/threonine protein kinase involved in regulating M phase functions during the cell cycle. May also be part of the signaling network controlling cellular adhesion. In vitro, is able to phosphorylate CDC25C and casein. -
Tissue specificity
Transcripts are highly detected in placenta, lung, followed by skeletal muscle, heart, pancreas, ovaries and kidney and weakly detected in liver and brain. May have a short half-live. In cells of hematopoietic origin, strongly and exclusively detected in terminally differentiated macrophages. Transcript expression appears to be down-regulated in primary lung tumor. -
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.
Contains 2 POLO box domains.
Contains 1 protein kinase domain. -
Post-translational
modificationsPhosphorylated as cells enter mitosis and dephosphorylated as cells exit mitosis. -
Cellular localization
Membrane. - Information by UniProt
Images
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The specific activity of PLK3 (ab125627) was determined to be 9 nmol/min/mg as per activity assay protocol and was equivalent to 2.2 nmol/min/mg as per radiometric assay
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SDS PAGE analysis of ab125627
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SDS-PAGe of Human PLK3 protein.
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The specific activity of ab125627 was determined to be 2.2 nmol/min/mg.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab125627 has not yet been referenced specifically in any publications.