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Question (31659) | Recombinant human SFRP1 protein (ab64445)

Hi, the description of the product ab64445 stated that it is glycosylated at 283 amino acid, can I please know what type of glycosylation it is and the size of the glycan? Thanks very much and looking forward to your reply. Best regards,

Thank you for your enquiry. I can confirm that the ab64445 SFRP1 protein is a glycosylated protein with a total of 283 amino acids and the glycosylation is located at residue 173. This particular product needs to be glycosylated in order for it to be active, which we have demonstrated by the activity tests. This product is also derived from HeLa cells as opposed to E.coli, so will be glycosylated. I am sorry we do not test for further details of the glycosylation, however, according to literature. it states the following: 'Analysis of glycosylation sites showed that sFRP-1 contains a relatively large carbohydrate moiety on Asn(173) (approximately 2.8 kDa).' I hope this will be helpful to you. If you have any additional questions, please do not hesitate to let me know.

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