Recombinant human VRK1 protein (ab125555)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 95% Densitometry
- Active: Yes
- Suitable for: WB, Functional Studies, SDS-PAGE
Description
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Product name
Recombinant human VRK1 protein -
Biological activity
The specific activity of ab125555 was determined to be 2 nmol/min/mg.
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Purity
> 95 % Densitometry.
Purity was determined to be >95% by densitometry. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Predicted molecular weight
71 kDa including tags -
Amino acids
1 to 396
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Specifications
Our Abpromise guarantee covers the use of ab125555 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
Functional Studies
SDS-PAGE
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Form
Liquid -
Additional notes
ab91090 (Cow Casein full length protein) can be utilized as a substrate for assessing Kinase activity
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol (glycerin, glycerine), 0.88% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- MGC117401
- MGC138280
- MGC142070
see all -
Function
Serine/threonine kinase that phosphorylates 'Thr-18' of p53/TP53 and may thereby prevent the interaction between p53/TP53 and MDM2. -
Tissue specificity
Widely expressed. Highly expressed in fetal liver, testis and thymus. -
Involvement in disease
Defects in VRK1 are the cause of pontocerebellar hypoplasia type 1 (PCH1) [MIM:607596]; also called pontocerebellar hypoplasia with infantile spinal muscular atrophy or pontocerebellar hypoplasia with anterior horn cell disease. PCH1 is characterized by an abnormally small cerebellum and brainstem, central and peripheral motor dysfunction from birth, gliosis and anterior horn cell degeneration resembling infantile spinal muscular atrophy (SMA). -
Sequence similarities
Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. VRK subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsAutophosphorylated at various serine and threonine residues. Autophosphorylation does not impair its ability to phosphorylate p53/TP53. -
Cellular localization
Nucleus. Dispersed throughout the cell but not located on mitotic spindle or chromatids during mitosis. - Information by UniProt
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab125555 has been referenced in 1 publication.
- Cartwright TN et al. Dissecting the roles of Haspin and VRK1 in histone H3 phosphorylation during mitosis. Sci Rep 12:11210 (2022). PubMed: 35778595