Recombinant mouse Wnt3a protein (ab81484)
Key features and details
- Expression system: Cell Culture
- Purity: > 75% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Suitable for: CellDiff, SDS-PAGE, Cellular Activation, HPLC
Description
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Product name
Recombinant mouse Wnt3a protein
See all Wnt3a proteins and peptides -
Biological activity
The ED50 was determined by its ability to induced alkaline phosphatase production by CCL-226 cells.
The expected ED50 for this effect is 0.4-0.6 ng/ml.
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Purity
> 75 % SDS-PAGE.
Greater than 75% by HPLC analyses. -
Endotoxin level
< 1.000 Eu/µg -
Expression system
Cell Culture -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Mouse -
Sequence
SY PIWWSLAVGP QYSSLSTQPI LCASIPGLVP KQLRFCRNYV EIMPSVAEGV KAGIQECQHQ FRGRRWNCTT VSNSLAIFGP VLDKATRESA FVHAIASAGV AFAVTRSCAE GSAAICGCSS RLQGSPGEGW KWGGCSEDIE FGGMVSREFA DARENRPDAR SAMNRHNNEA GRQAIASHMH LKCKCHGLSG SCEVKTCWWS QPDFRTIGDF LKDKYDSASE MVVEKHRESR GWVETLRPRY TYFKVPTERD LVYYEASPNF CEPNPETGSF GTRDRTCNVS SHGIDGCDLL CCGRGHNART ERRREKCHCV FHWCCYVSCQ ECTRVYDVHT CK -
Amino acids
19 to 352 -
Additional sequence information
ab81484 is a monomeric glycoprotein containing 334 amino acid residues. This protein is the mature full length (aa19-352), it does not contain the signal peptide from aa 1-18.
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab81484 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Cell Differentiation
SDS-PAGE
Cellular Activation
HPLC
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Form
Lyophilized -
Additional notes
Endotoxin level is less than 0.2 ng per µg (2EU/µg).
Formulation: Sterile filtered through a 0.2 micron filter. Lyophilized from 10mM Sodium Phosphate, pH 7.5 + 500mM NaCl + 0.5% CHAPS.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at 4°C (up to 6 months). The lyophilized protein is stable for a few weeks at room temperature. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituent: 13% CHAPS
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute to a concentration of 0.1 mg/mL with ddH2O. Store at 4oC for up to 1 week or dilute further in a buffer containing a carrier protein or stabilizer and store working aliquots at -20oC to -80oC for up to 3 months.
General Info
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Alternative names
- Protein Wnt 3a Precursor
- Protein Wnt-3a
- Wingless type MMTV integration site family member 3A
see all -
Function
Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube. -
Tissue specificity
Moderately expressed in placenta and at low levels in adult lung, spleen, and prostate. -
Sequence similarities
Belongs to the Wnt family. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
Images
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Human embryonic stem cells (HUES4) were differentiated as monolayer cultures on matrigel coated dishes using a protocol described in Kroon et al, Nat Biotechnol. (2008). Inhibin beta A (ab50051) and Wnt3a (ab81484) were added to cultures to direct differentiation towards definitive endoderm. This image was taken 3 days after induction, where the definitive endoderm marker FOXA2 (ab40874 in green) is abundant. Dapi staining is shown in blue. Magnification: 200x. Negative controls of fluorescently conjugated secondary antibodies only showed no staining.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (15)
ab81484 has been referenced in 15 publications.
- Balbasi E et al. Directed Differentiation of Mouse Embryonic Stem Cells to Mesoderm, Endoderm, and Neuroectoderm Lineages. Methods Mol Biol 2520:295-307 (2022). PubMed: 34611822
- Cheng K et al. Targeted intestinal deletion of Rho guanine nucleotide exchange factor 7, ßPIX, impairs enterocyte proliferation, villus maturation, and mucosal defenses in mice. Am J Physiol Gastrointest Liver Physiol 320:G627-G643 (2021). PubMed: 33566751
- Liu B et al. Loss of endothelial glucocorticoid receptor promotes angiogenesis via upregulation of Wnt/ß-catenin pathway. Angiogenesis 24:631-645 (2021). PubMed: 33650028
- Gong Q et al. Fractalkine aggravates LPS-induced macrophage activation and acute kidney injury via Wnt/ß-catenin signalling pathway. J Cell Mol Med 25:6963-6975 (2021). PubMed: 34101346
- Zhou C et al. LncRNA PVT1 promotes gemcitabine resistance of pancreatic cancer via activating Wnt/ß-catenin and autophagy pathway through modulating the miR-619-5p/Pygo2 and miR-619-5p/ATG14 axes. Mol Cancer 19:118 (2020). PubMed: 32727463