Recombinant rat BVR protein (ab91622)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: WB, Functional Studies, SDS-PAGE
Description
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Product name
Recombinant rat BVR protein
See all BVR proteins and peptides -
Biological activity
BVR Assay: Positive (> 180 units/mg) 1 unit of BVR will transform 1 nanomole of biliverdin to bilirubin in an NADPH dependent reaction at pH 8.5 at 37°C in 1 minute.
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Purity
> 95 % SDS-PAGE.
Purified by Multi-Step Chromatography. > 95% pure as determined by SDS-PAGE and Western Blot analysis. -
Expression system
Escherichia coli -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Rat
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Specifications
Our Abpromise guarantee covers the use of ab91622 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
Functional Studies
SDS-PAGE
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Form
Liquid -
Additional notes
BVR Assay: Positive (> 180 units/mg) 1 unit of BVR will transform 1 nanomole of biliverdin to bilirubin in an NADPH dependent reaction at pH 8.5 at 37°C in 1 minute.
Protein previously labeled as Biliverdin Reductase.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 0.077% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.79% Tris HCl, 0.0292% EDTA
This product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- BIEA_HUMAN
- Biliverdin IX alpha reductase
- Biliverdin reductase A
see all -
Function
Reduces the gamma-methene bridge of the open tetrapyrrole, biliverdin IX alpha, to bilirubin with the concomitant oxidation of a NADH or NADPH cofactor. -
Tissue specificity
Liver. -
Pathway
Porphyrin-containing compound metabolism; protoheme degradation. -
Involvement in disease
Hyperbiliverdinemia -
Sequence similarities
Belongs to the Gfo/Idh/MocA family. Biliverdin reductase subfamily. -
Cellular localization
Cytoplasm. - Information by UniProt
Images
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SDS-PAGE Analysis:
Lane 1: Molecular weight marker Lane 2: 0.5 µg ab91622
Lane 3: 1.0 µg ab91622 Lane 4: 2.0 µg ab91622
Lane 5: 5.0 µg ab91622 -
All lanes : BVR Polyclonal Antibody
Lane 1 : Molecular weight markers
Lane 2 : ab91622 at 0.1 µg -
BVR Activity: 1 µg of ab91622 was incubated with biliverdin in the presence of NADPH at pH 8.5, 37°C. Product formation was monitored with a spectrometer at 450 nm.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab91622 has not yet been referenced specifically in any publications.