Recoverin Human ELISA Kit (ab108898)
- Product nameRecoverin Human ELISA Kit
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma, Tissue
- Assay typeSandwich
- Sensitivity= 50 pg/ml
- Range0.078 ng/ml - 20 ng/ml
- Species reactivityReacts with: Human
- Product overview
Recoverin is a calcium-dependent inhibitor of rhodopsin kinase, a member of the EF-hand family of calcium-binding proteins involved in the transduction of light by vertebrate photoreceptors. Recoverin also was identified as an autoantigen in the degenerative disease of the retina known as cancer-associated retinopathy (CAR), a paraneoplastic syndrome whereby immunological events lead to the degeneration of photoreceptors in some individuals with cancer.
ab108898 Recoverin Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of Human Recoverin in plasma, serum, tissue and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures Recoverin in 3.5 hours. A polyclonal antibody specific for Recoverin has been pre-coated onto a microplate. Recoverin in standards and samples is sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for Recoverin, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
- Tested applicationsSandwich ELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests Biotinylated Recoverin Antibody 1 x 80µl Chromogen Substrate 1 x 8ml Diluent Concentrate (10x) 1 x 30ml Recoverin Microplate (12 strips of 8 wells) 1 unit Recoverin Standard 1 unit Sealing Tapes 1 unit Stop Solution 1 x 12ml Streptavidin-Peroxidase Conjugate (SP Conjugate) 1 x 80µl Wash Buffer Concentrate (20x) 1 x 30ml
Contains 4 EF-hand domains.
- Cancer-associated retinopathy proteinProtein CARRCVRN
Our Abpromise guarantee covers the use of ab108898 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for Recoverin Human ELISA Kit (ab108898)
ab108898 has not yet been referenced specifically in any publications.