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Overview

  • Product nameRecoverin Human ELISA Kit
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma, Tissue
  • Assay typeSandwich
  • Sensitivity
    = 50 pg/ml
  • Range
    0.078 ng/ml - 20 ng/ml
  • Species reactivity
    Reacts with: Human
  • Product overview

    Recoverin is a calcium-dependent inhibitor of rhodopsin kinase, a member of the EF-hand family of calcium-binding proteins involved in the transduction of light by vertebrate photoreceptors. Recoverin also was identified as an autoantigen in the degenerative disease of the retina known as cancer-associated retinopathy (CAR), a paraneoplastic syndrome whereby immunological events lead to the degeneration of photoreceptors in some individuals with cancer.

    ab108898 Recoverin Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of Human Recoverin in plasma, serum, tissue and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures Recoverin in 3.5 hours. A polyclonal antibody specific for Recoverin has been pre-coated onto a microplate. Recoverin in standards and samples is sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for Recoverin, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

  • Tested applicationsSandwich ELISA more details

Properties

  • FunctionSeems to be implicated in the pathway from retinal rod guanylate cyclase to rhodopsin. May be involved in the inhibition of the phosphorylation of rhodopsin in a calcium-dependent manner. The calcium-bound recoverin prolongs the photoresponse.
  • Tissue specificityRetina and pineal gland.
  • Sequence similaritiesBelongs to the recoverin family.
    Contains 4 EF-hand domains.

    • Target information above from: UniProt accession P35243 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        Cancer-associated retinopathy proteinProtein CARRCVRN
        RECO_HUMANRecoverin
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab108898 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      Sandwich ELISA sELISA

      Recoverin Human ELISA Kit images

      • Sandwich ELISA - Recoverin Human ELISA Kit -1 x 96 Well Plate (ab108898)
        Sandwich ELISA - Recoverin Human ELISA Kit -1 x 96 Well Plate (ab108898)

      Protocols

      References for Recoverin Human ELISA Kit (ab108898)

      ab108898 has not yet been referenced specifically in any publications.

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"