Anti-Rel B antibody (ab71543)
- Product nameAnti-Rel B antibodySee all Rel B primary antibodies ...
- DescriptionRabbit polyclonal to Rel B
- Tested applicationsWB more details
- Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide: REAAFGGGLLSPGPEAT, corresponding to amino acids 563-579 of Rel B
- Positive control
- HuT78, L929, C6 or NIH3T3 nuclear lysate
- Storage instructionsStore at -20°C. Stable for 12 months at -20°C
- Storage bufferPreservative: 0.03% Sodium Azide
Constituents: 50% Glycerol, 0.01% BSA, HEPES, 0.15M Sodium chloride
- PurityWhole antiserum
- Clonality Polyclonal
Our Abpromise guarantee covers the use of ab71543 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||WB: 1/2000. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).|
- FunctionNF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric RelB-p50 and RelB-p52 complexes are transcriptional activators. RELB neither associates with DNA nor with RELA/p65 or REL. Stimulates promoter activity in the presence of NFKB2/p49.
- Sequence similaritiesContains 1 RHD (Rel-like) domain.
- DomainBoth N- and C-terminal domains are required for transcriptional activation.
modificationsPhosphorylation at 'Thr-103' and 'Ser-573' is followed by proteasomal degradation.
- Cellular localizationNucleus. Cytoplasm > cytoskeleton > centrosome. Co-localizes with NEK6 in the centrosome.
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Anti-Rel B antibody images
All lanes : Anti-Rel B antibody (ab71543) at 1/2000 dilution
Lane 1 : HuT78 cell lysate
Lane 2 : L929 cell lysate
Lane 3 : C6 cell lysate
Lane 4 : NIH3T3 cell lysate
Predicted band size : 62 kDa
Observed band size : 62 kDa
References for Anti-Rel B antibody (ab71543)
ab71543 has not yet been referenced specifically in any publications.