Anti-Renalase antibody (ab31291)
Key features and details
- Goat polyclonal to Renalase
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-Renalase antibody
See all Renalase primary antibodies -
Description
Goat polyclonal to Renalase -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Dog -
Immunogen
Synthetic peptide corresponding to Human Renalase aa 200-300 (internal sequence).
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab31291 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).
1 hour primary incubation at room temperature is recommended for this product. |
Notes |
---|
WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa). 1 hour primary incubation at room temperature is recommended for this product. |
Target
-
Function
Probable FAD-dependent amine oxidase secreted by the kidney, which circulates in blood and modulates cardiac function and systemic blood pressure. Degrades catecholamines such as dopamine, norepinephrine and epinephrine in vitro. Lowers blood pressure in vivo by decreasing cardiac contractility and heart rate and preventing a compensatory increase in peripheral vascular tone, suggesting a causal link to the increased plasma catecholamine and heightened cardiovascular risk. High concentrations of catecholamines activate plasma renalase and promotes its secretion and synthesis (By similarity). According to PubMed:17385068, is unlikely that renalase has physiologically relevant catecholamine-oxidizing activity. -
Tissue specificity
Secreted into the blood by the kidney. Highly expressed in the kidney, expressed at lower level in heart, skeletal muscle and small intestine. Its plasma concentration is markedly reduced in patients with end-stage renal disease, as compared with healthy subjects. -
Sequence similarities
Belongs to the renalase family. -
Cellular localization
Secreted. - Information by UniProt
-
Database links
- Entrez Gene: 55328 Human
- Entrez Gene: 67795 Mouse
- Entrez Gene: 361751 Rat
- Omim: 609360 Human
- SwissProt: Q5VYX0 Human
- SwissProt: A7RDN6 Mouse
- SwissProt: Q5U2W9 Rat
- Unigene: 149849 Human
see all -
Alternative names
- 6530404N21Rik antibody
- AI452315 antibody
- AW060440 antibody
see all
Images
Protocols
Datasheets and documents
-
Datasheet download
References (9)
ab31291 has been referenced in 9 publications.
- Chang J et al. Identification of Two Forms of Human Plasma Renalase, and Their Association With All-Cause Mortality. Kidney Int Rep 5:362-368 (2020). PubMed: 32154458
- Safdar B et al. Elevated renalase levels in patients with acute coronary microvascular dysfunction - A possible biomarker for ischemia. Int J Cardiol 279:155-161 (2019). PubMed: 30630613
- Hollander L et al. Renalase Expression by Melanoma and Tumor-Associated Macrophages Promotes Tumor Growth through a STAT3-Mediated Mechanism. Cancer Res 76:3884-94 (2016). PubMed: 27197188
- Guo X et al. Inhibition of renalase expression and signaling has antitumor activity in pancreatic cancer. Sci Rep 6:22996 (2016). PubMed: 26972355
- Qi C et al. Serum Renalase Levels Correlate with Disease Activity in Lupus Nephritis. PLoS One 10:e0139627 (2015). PubMed: 26431044
- Wang F et al. Renalase contributes to the renal protection of delayed ischaemic preconditioning via the regulation of hypoxia-inducible factor-1a. J Cell Mol Med 19:1400-9 (2015). WB . PubMed: 25781495
- Wang F et al. Epinephrine evokes renalase secretion via a-adrenoceptor/NF-?B pathways in renal proximal tubular epithelial cells. Kidney Blood Press Res 39:252-9 (2014). PubMed: 25171187
- Lee HT et al. Renalase protects against ischemic AKI. J Am Soc Nephrol 24:445-55 (2013). WB ; Mouse . PubMed: 23393318
- Pandini V et al. Synthesis of human renalase1 in Escherichia coli and its purification as a FAD-containing holoprotein. Protein Expr Purif 72:244-53 (2010). WB ; Human . PubMed: 20302943