You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Reassurance, Refunds & Replacements

If your product does not perform as described on this datasheet, we will refund or replace your product...

Read our guarantee »
 

Anti-Repulsive Guidance Molecule C antibody [Fery-1] (ab54431)

CodeSizePriceAbpointsAvailability
    
Add to basket
 
  • -
  •   
  •   
  •   
  •  
  •   
  •   
Updating...

Overview

Product name

Anti-Repulsive Guidance Molecule C antibody [Fery-1]
See all Repulsive Guidance Molecule C products (4) ...

Description

Mouse monoclonal [Fery-1] to Repulsive Guidance Molecule C

Specificity

ab54431 recognizes human Repulsive Guidance Molecule C.

Tested applications

IP, Flow Cyt, WBmore details

Cross reactivity

Reacts with

Mouse, Human

Immunogen

Recombinant fragment, corresponding to amino acids 36-398 of Human Repulsive Guidance Molecule C

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: PBS

Concentration

Concentration information loading...

Purity

Protein G purified

Purification notes

Purified from concentrated hybridoma tissue cell culture supernatant. Greater than 95% pure by SDS-PAGE.

Clonality

Monoclonal

Clone number

Fery-1

Isotype

IgG2b

Research areas

Developmental Biology >> Lineage specification >> Mesoderm
Signal Transduction >> Metabolism >> Vitamins / Minerals
Stem Cells >> Lineage Markers >> Mesoderm
Neuroscience >> Neurology process >> Neurogenesis

  • Western blot - Repulsive Guidance Molecule C antibody [Fery-1] (ab54431)Western blot - Repulsive Guidance Molecule C antibody [Fery-1] (ab54431) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab54431 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

Mutations in Repulsive Guidance Molecule (also known as RGM-C; HJV; hemojuvelin; Hfe2) cause juvenile hemochromatosis, a severe iron overload disease. RGM-C gene expression has been characterized in the developing mouse and found to be exclusively expressed in all striated muscle and in the myocardium.

Cellular localization

Cell membrane; Lipid-anchor, GPI-anchor

Alternative names

  • 2310035L15Rik antibody
  • 5230400G09Rik antibody
  • AI414844 antibody
  • AI789733 antibody
  • DL M antibody
  • Haemojuvelin antibody
  • HEMOCHROMATOSIS antibody
  • HEMOCHROMATOSIS DUE TO DEFECT IN HEMOJUVELIN antibody
  • HEMOCHROMATOSIS DUE TO DEFECT IN HEPCIDIN ANTIMICROBIAL PEPTIDE antibody
  • HEMOCHROMATOSIS JUVENILE antibody
  • Hemochromatosis type 2 (juvenile) antibody
  • Hemochromatosis type 2 antibody
  • Hemochromatosis type 2 protein antibody
  • Hemochromatosis type 2 protein homolog antibody
  • HEMOCHROMATOSIS, TYPE 2A antibody
  • HEMOCHROMATOSIS, TYPE 2B antibody
  • Hemojuvelin antibody
  • HFE 2 antibody
  • Hfe2 antibody
  • HFE2A antibody
  • HJV antibody
  • JH antibody
  • Juvenile antibody
  • MGC23953 antibody
  • OTTHUMP00000059680 antibody
  • RGM C antibody
  • RGM domain family member C antibody
  • RGMC antibody
see all

Database links

Anti-Repulsive Guidance Molecule C antibody [Fery-1] images:

  Western blot - Repulsive Guidance Molecule C antibody [Fery-1] (ab54431)

Western blot - Repulsive Guidance Molecule C antibody [Fery-1] (ab54431)

All lanes : Anti-Repulsive Guidance Molecule C antibody [Fery-1] (ab54431) at 1/1000 dilution

Lane 1 : embryonic day 16 mouse muscle
Lane 2 : adult mouse heart

Lysates/proteins at 20 µg per lane.

Secondary
HRP conjugated at 1/3000 dilution
developed using the ECL technique

Performed under reducing conditions.

Predicted band size : 50, 37 kDa
Additional bands at : 45&74&100 kDa (possible non-specific binding).

Exposure time : 2 minutes

Ruma Raha-Chowdhury, University Of Cambridge, United Kingdom

See Abreview

References for Anti-Repulsive Guidance Molecule C antibody [Fery-1] (ab54431)

This product has been referenced in:

  • Lakhal Set al. Regulation of Type II Transmembrane Serine Proteinase TMPRSS6 by Hypoxia-inducible Factors: NEW LINK BETWEEN HYPOXIA SIGNALING AND IRON HOMEOSTASIS. J Biol Chem 286:4090-7 (2011). Flow Cyt; Human.Read more (PubMed: 20966077) »

See 1 publication for this product

Publishing research using ab54431? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"