Resistin Human ELISA Kit (ab108896)
Overview
- Product nameResistin Human ELISA KitSee all Resistin kits ...
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Urine, Serum, Plasma
- Assay typeSandwich
- Sensitivity= 0.2 ng/ml
- Range0.25 ng/ml - 16 ng/ml
- Recovery
= 99.5 %
- Assay time5h 00m
- Species reactivityReacts with: Human
- Product overview
Resistin, a novel adipose-derived protein, has been proposed to cause insulin-resistant states in obesity. Resistin is produced by white and brown adipose tissues but has also has been identified in several other tissues, including the hypothalamus, pituitary and adrenal glands, pancreas, gastrointestinal tract, myocytes, spleen, white blood cells and plasma. Resistin antagonizes insulin action, and is down regulated by rosiglitazone and peroxisome proliferator-activated receptor gamma agonists. Resistin is elevated in patients with Type II diabetes and may play a role in the vascular complications of this disorder.
ab108896 Resistin Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of Human Resistin in plasma, serum, urine, and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique, which measures Resistin in less than 5 hours. A Murine monoclonal antibody specific for Resistin has been pre-coated onto a microplate. Resistin standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for Resistin, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
- Tested applicationsSandwich ELISA more details
Properties
- Storage instructionsStore at +4°C. Please refer to protocols.
-
Components 1 x 96 tests Biotinylated Human Resistin Antibody 1 x 80µl Chromogen Substrate 1 x 8ml Diluent Concentrate (10x) 1 x 30ml Human Resistin Microplate 1 x 96 wells 1 unit Human Resistin Standard 1 unit Stop Solution 1 x 12ml Streptavidin-Peroxidase Conjugate (SP Conjugate) 1 x 80µl Wash Buffer Concentrate (20x) 1 x 30ml - Research Areas
Target information above from: UniProt accession
Q9HD89
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
.
-
Alternative names
- Adipose tissue specific secretory factor Adipose tissue-specific secretory factorADSF
- C/EBP epsilon regulated myeloid specific secreted cysteine rich proteinC/EBP epsilon regulated myeloid specific secreted cysteine rich protein precursor 1 C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich proteinCysteine rich secreted protein A12 alpha like 2 Cysteine rich secreted protein FIZZ3Cysteine-rich secreted protein A12-alpha-like 2Cysteine-rich secreted protein FIZZ3FIZZ 3FIZZ3Found in inflammatory zone 3HXCP 1HXCP1MGC126603MGC126609PRO1199ResistinResistin delta2 RETNRETN 1RETN_HUMANRETN1UNQ407 XCP 1 XCP1
see all
- Entrez Gene: 56729 Human
- Omim: 605565 Human
- SwissProt: Q9HD89 Human
- Unigene: 283091 Human
Applications
Our Abpromise guarantee covers the use of ab108896 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Sandwich ELISA | sELISA |
Resistin Human ELISA Kit images
-
ELISA - Resistin Human ELISA Kit - 1 x 96 Well Plate (ab108896)Human Resistin standard curve.
Protocols
References for Resistin Human ELISA Kit (ab108896)
ab108896 has not yet been referenced specifically in any publications.
