Anti-Respiratory Syncytial Virus antibody (ab20745)

Overview

  • Product nameAnti-Respiratory Syncytial Virus antibody
    See all Respiratory Syncytial Virus primary antibodies
  • Description
    Goat polyclonal to Respiratory Syncytial Virus
  • SpecificityAll viral antigens including RSV A and RSV B. Does not react with Parainfluenza 1 to 3, Influenza A and B or Adenovirus by IFA. Negative cross reactivity with HEp2 cells and WI38 cells. Reacts well with bovine RSV isolates.
  • Tested applicationsICC/IF, IHC-P, WB, ELISA, Conjugationmore details
  • Species reactivity
    Reacts with RSV A and RSV B. Not yet tested in other species.
  • Immunogen

    Viral lysate of Human RSV isolate.

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: 0.01M PBS, pH 7.2
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesSodium sulphate precipitation and DEAE chromatography.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab20745 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution. PubMed: 20647383
IHC-P Use at an assay dependent dilution.
WB Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.
Conjugation Use at an assay dependent dilution.

Target

  • RelevanceRespiratory syncytial virus (RSV) is a major cause of respiratory illness in young children. RSV infection produces a variety of signs and symptoms involving different areas of the respiratory tract, from the nose to the lungs. RSV is a negative sense, enveloped RNA virus. The virion is variable in shape and size with average diameter of between 120 and 300 nm. The 63 kD RSV fusion protein of the RSS 2 strain (subtype A) directs fusion of viral and cellular membranes, results in viral penetration, and can direct fusion of infected cells with adjoining cells, resulting in the formation of syncytia or multi nucleated giant cells.
  • Cellular localizationVirion. Host cytoplasm
  • Alternative names
    • RS virus antibody
    • RSV antibody

Anti-Respiratory Syncytial Virus antibody images

  • Immunofluorescence analysis of A549 cells infected with RSV, staining Respiratory Syncytial Virus with ab20745.

    Cells were fixed with formalin before staining with primary antibody, followed by ab6881 - Donkey polyclonal Secondary Antibody to Goat IgG - H&L (FITC).

References for Anti-Respiratory Syncytial Virus antibody (ab20745)

This product has been referenced in:
  • Smith CM  et al. Ciliary dyskinesia is an early feature of respiratory syncytial virus infection. Eur Respir J 43:485-96 (2014). ICC/IF . Read more (PubMed: 23520320) »
  • Satkunanathan S  et al. Respiratory syncytial virus infection, TLR3 ligands, and proinflammatory cytokines induce CD161 ligand LLT1 expression on the respiratory epithelium. J Virol 88:2366-73 (2014). ICC/IF . Read more (PubMed: 24352438) »

See all 10 Publications for this product

Product Wall

Thank you for your enquiry. According to our laboratory notes we would recommend a ProteinG beads system for immunoprecipitation of goat antibodies. I hope this information helps. Please do not hesitate to contact us if you need any more advice...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"