Anti-Retinoic Acid Receptor alpha antibody [EP1823Y] (ab76074)

Overview

• Product nameAnti-Retinoic Acid Receptor alpha antibody [EP1823Y]See all Retinoic Acid Receptor alpha primary antibodies ...
• Description
  Rabbit monoclonal [EP1823Y] to Retinoic Acid Receptor alpha
• Tested applicationsWB, Flow Cyt more details
• Species reactivity
  Reacts with: Mouse, Human
  

  Does not react with

  Rat
• Immunogen

  Synthetic peptide corresponding to residues near the N-terminal of human Retinoic Acid Receptor alpha.

• Positive controlPermeabilized MCF-7 cells. MCF-7 cell lysate.
• General notesProduced under U.S. Patent No. 5,675,063.

Properties

• FormLiquid
• Storage instructionsStore at -20°C. Stable for 12 months at -20°C
• Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• PurityTissue culture supernatant
• Clonality Monoclonal
• Clone numberEP1823Y
• IsotypeIgG
• Research Areas
  • Cancer
  • Signal transduction
  • Nuclear signaling
  • Nuclear hormone receptors
  • Retanoic and retanoid

  • Signal Transduction
  • Signaling Pathway
  • Nuclear Signaling
  • Nuclear Hormone Receptors
  • Retinoic & Retinoid

  • Epigenetics and Nuclear Signaling
  • Nuclear Signaling Pathways
  • Nuclear Receptors
  • Retinoic & Retinoid

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Domain Families
  • Zinc Finger

Applications

Target

• FunctionReceptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone acetylation, chromatin condensation and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. RARA plays an essential role in the regulation of retinoic acid-induced germ cell development during spermatogenesis. Has a role in the survival of early spermatocytes at the beginning prophase of meiosis. In Sertoli cells, may promote the survival and development of early meiotic prophase spermatocytes. In concert with RARG, required for skeletal growth, matrix homeostasis and growth plate function (By similarity). Regulates expression of target genes in a ligand-dependent manner by recruiting chromatin complexes containing MLL5. Mediates retinoic acid-induced granulopoiesis.
• Involvement in diseaseNote=Chromosomal aberrations involving RARA are commonly found in acute promyelocytic leukemia. Translocation t(11;17)(q32;q21) with ZBTB16/PLZF; translocation t(15;17)(q21;q21) with PML; translocation t(5;17)(q32;q11) with NPM. The PML-RARA oncoprotein requires both the PML ring structure and coiled-coil domain for both interaction with UBE2I, nuclear microspeckle location and sumoylation. In addition, the coiled-coil domain functions in blocking RA-mediated transactivation and cell differentiation.
• Sequence similaritiesBelongs to the nuclear hormone receptor family. NR1 subfamily.
  Contains 1 nuclear receptor DNA-binding domain.
• DomainComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
• Post-translational
  modificationsPhosphorylated on serine and threonine residues. Phosphorylation does not change during cell cycle. Phosphorylation on Ser-77 is crucial for transcriptional activity (By similarity). Phosphorylation by AKT1 is required for the repressor activity but has no effect on DNA binding, protein stability nor subcellular localization. Phosporylated by PKA in vitro. This phosphorylation on Ser-219 and Ser-369 is critical for ligand binding, nuclear localization and transcriptional activity in response to FSH signaling.
  Sumoylated by SUMO2, mainly on Lys-399 which is also required for SENP6 binding. On all-trans retinoic acid (ATRA) binding, a confromational change may occur that allows sumoylation on two additional site, Lys-166 and Lys-171. Probably desumoylated by SENP6. Sumoylation levels determine nuclear localization and regulate ATRA-mediated transcriptional activity.
  Trimethylation enhances heterodimerization with RXRA and positively modulates the transcriptional activation.
  Ubiquitinated.
• Cellular localizationNucleus. Cytoplasm. Nuclear localization depends on ligand binding, phosphorylation and sumoylation. Transloaction to the nucleus in the absence of ligand is dependent on activation of PKC and the downstream MAPK phosphorylation.

Target information above from: UniProt accession P10276 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 5914 Human
  • Entrez Gene: 19401 Mouse
  • Omim: 180240 Human
  • SwissProt: P10276 Human
  • SwissProt: P11416 Mouse
  • Unigene: 654583 Human
  • Unigene: 439744 Mouse

• Alternative names
  Acute Promelocytic Leukemia Breakpoint Cluster Region antibodyNR1B1 antibodyNuclear mitotic apparatus protein retinoic acid receptor alpha fusion protein antibody

  Nuclear receptor subfamily 1 group B member 1 antibodyNucleophosmin retinoic acid receptor alpha fusion protein NPM RAR long form antibodyNuMA RARA fusion antibodyNuMARARA fusion antibodyOTTHUMP00000164454 antibodyOTTHUMP00000164456 antibodyRAR A antibodyRAR alpha antibodyRAR alpha form antibodyRAR antibodyRAR-alpha antibodyRARA antibodyRARA protein antibodyRARA/NUMA1 Fusion Gene antibodyRARA/PLZF Fusion Gene antibodyRARA/PML Fusion Gene antibodyRARA/PRKAR1A Fusion Gene antibodyRARA_HUMAN antibodyRARalpha antibodyRARalpha1 antibodyRetinoic acid receptor alpha antibodyRetinoic acid receptor alpha polypeptide antibody

  see all

Anti-Retinoic Acid Receptor alpha antibody [EP1823Y] images

• 

  Flow Cytometry - Retinoic Acid Receptor alpha antibody [EP1823Y] (ab76074)
  Flow cytometric analysis of permeabilized MCF-7 cells using ab76074 at 1/50 dilution (red) or a rabbit IgG (negative) (green).
• 

  Western blot - Retinoic Acid Receptor alpha antibody [EP1823Y] (ab76074)
  Anti-Retinoic Acid Receptor alpha antibody [EP1823Y] (ab76074) at 1/1000 dilution + MCF-7 cell lysate at 10 µg
  
  Predicted band size : 51 kDa
  Observed band size : 55 kDa (why is the actual band size different from the predicted?)