Anti-Retinoic Acid Receptor beta antibody (ab53161)

Overview

• Product nameAnti-Retinoic Acid Receptor beta antibodySee all Retinoic Acid Receptor beta primary antibodies ...
• Description
  Rabbit polyclonal to Retinoic Acid Receptor beta
• Tested applicationsWB, ELISA, IHC-P, IP, ICC/IF more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  Synthetic peptide, corresponding to amino acids 351-390 of Human Retinoic Acid Receptor beta

• Positive controlHuman breast carcinoma tissue HepG2 cell extract

Properties

• FormLiquid
• Storage instructionsStore at -20°C. Stable for 12 months at -20°C
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Cancer
  • Signal transduction
  • Nuclear signaling
  • Nuclear hormone receptors
  • Retanoic and retanoid

  • Signal Transduction
  • Signaling Pathway
  • Nuclear Signaling
  • Nuclear Hormone Receptors
  • Retinoic & Retinoid

  • Epigenetics and Nuclear Signaling
  • Nuclear Signaling Pathways
  • Nuclear Receptors
  • Retinoic & Retinoid

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Domain Families
  • Zinc Finger

Applications

Target

• FunctionReceptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence or presence of hormone ligand, acts mainly as an activator of gene expression due to weak binding to corepressors. In concert with RARG, required for skeletal growth, matrix homeostasis and growth plate function.
• Sequence similaritiesBelongs to the nuclear hormone receptor family. NR1 subfamily.
  Contains 1 nuclear receptor DNA-binding domain.
• DomainComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
• Cellular localizationCytoplasm and Nucleus.

Target information above from: UniProt accession P10826 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 5915 Human
  • Entrez Gene: 218772 Mouse
  • Entrez Gene: 24706 Rat
  • Omim: 180220 Human
  • SwissProt: P10826 Human
  • SwissProt: P22605 Mouse
  • Unigene: 654490 Human
  • Unigene: 654490 Human

  • Unigene: 259318 Mouse
  • Unigene: 220045 Rat

  see all

• Alternative names
  HAP antibodyHBV activated protein antibodyHBV-activated protein antibody

  Hepatitis B virus activated protein antibodyNR1B2 antibodyNuclear receptor subfamily 1 group B member 2 antibodyRAR B antibodyRAR beta antibodyRAR epsilon antibodyRAR-beta antibodyRAR-epsilon antibodyRARB antibodyRARB_HUMAN antibodyRetinoic acid receptor beta 2 antibodyRetinoic acid receptor beta 4 antibodyRetinoic acid receptor beta 5 antibodyRetinoic acid receptor beta antibodyRetinoic acid receptor beta polypeptide antibodyRRB 2 antibodyRRB2 antibody

  see all

Anti-Retinoic Acid Receptor beta antibody images

• 

  Immunohistochemistry (Paraffin-embedded sections) - Retinoic Acid Receptor beta antibody (ab53161)
  This image shows human breast carcinoma tissue stained with ab53161 at 1/50 dilution. The left hand image shows untreated tissue; the right hand image shows tissue treated with the immunising peptide.
• 

  Western blot - Retinoic Acid Receptor beta antibody (ab53161)
  All lanes : Anti-Retinoic Acid Receptor beta antibody (ab53161) at 1/500 dilution
  
  Lane 1 : HepG2 cell extract, untreated.
  Lane 2 : HepG2 cell extract treated with the immunising peptide.
  
  
  Predicted band size : 50 kDa
  Observed band size : 50 kDa
  
• 

  Immunocytochemistry/ Immunofluorescence-Retinoic Acid Receptor beta antibody(ab53161)
  ICC/IF image of ab53161 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53161, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.