Anti-Retinoic Acid Receptor beta antibody (ab53161)

Overview

  • Product nameAnti-Retinoic Acid Receptor beta antibodySee all Retinoic Acid Receptor beta primary antibodies ...
  • Description
    Rabbit polyclonal to Retinoic Acid Receptor beta
  • Tested applicationsWB, ELISA, IHC-P, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to amino acids 351-390 of Human Retinoic Acid Receptor beta

  • Positive control
    • Human breast carcinoma tissue HepG2 cell extract

Properties

Applications

Our Abpromise guarantee covers the use of ab53161 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
ELISA 1/20000.
IHC-P Use at an assay dependent dilution.
IP Use at an assay dependent dilution. PubMed: 20655472
ICC/IF Use a concentration of 1 - 5 µg/ml.

Target

  • FunctionReceptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence or presence of hormone ligand, acts mainly as an activator of gene expression due to weak binding to corepressors. In concert with RARG, required for skeletal growth, matrix homeostasis and growth plate function.
  • Sequence similaritiesBelongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • DomainComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • Cellular localizationCytoplasm and Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • HAP antibody
    • HBV activated protein antibody
    • HBV-activated protein antibody
    • Hepatitis B virus activated protein antibody
    • NR1B2 antibody
    • Nuclear receptor subfamily 1 group B member 2 antibody
    • RAR B antibody
    • RAR beta antibody
    • RAR epsilon antibody
    • RAR-beta antibody
    • RAR-epsilon antibody
    • RARB antibody
    • RARB_HUMAN antibody
    • Retinoic acid receptor beta 2 antibody
    • Retinoic acid receptor beta 4 antibody
    • Retinoic acid receptor beta 5 antibody
    • Retinoic acid receptor beta antibody
    • Retinoic acid receptor beta polypeptide antibody
    • RRB 2 antibody
    • RRB2 antibody
    see all

Anti-Retinoic Acid Receptor beta antibody images

  • This image shows human breast carcinoma tissue stained with ab53161 at 1/50 dilution. The left hand image shows untreated tissue; the right hand image shows tissue treated with the immunising peptide.
  • All lanes : Anti-Retinoic Acid Receptor beta antibody (ab53161) at 1/500 dilution

    Lane 1 : HepG2 cell extract, untreated.
    Lane 2 : HepG2 cell extract treated with the immunising peptide.


    Predicted band size : 50 kDa
    Observed band size : 50 kDa
  • ICC/IF image of ab53161 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53161, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Retinoic Acid Receptor beta antibody (ab53161)

This product has been referenced in:
  • Marzese DM  et al. DNA methylation and gene deletion analysis of brain metastases in melanoma patients identifies mutually exclusive molecular alterations. Neuro Oncol N/A:N/A (2014). WB ; Human . Read more (PubMed: 24968695) »
  • Joss-Moore L  et al. Intrauterine growth restriction transiently delays alveolar formation and disrupts retinoic acid receptor expression in the lung of female rat pups. Pediatr Res 73:612-20 (2013). Rat . Read more (PubMed: 23419538) »

See all 4 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (adult testis)
Specification adult testis
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako antigen retrieval solution
Permeabilization No
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Zachary Yu-Ching Lin

Verified customer

Submitted Jan 18 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Marmoset (common) Tissue sections (marmoset adult testis section)
Specification marmoset adult testis section
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako antigen retrieval solution
Permeabilization No
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Zachary Yu-Ching Lin

Verified customer

Submitted Jan 18 2013

Thank you for your enquiry. I am sorry to hear that you are experiencing difficulties with this product ab53161 in western blot. Often it is possible to make suggestions that help resolve problems. We will happily offer technical support and in the ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"