Anti-Retinoic Acid Receptor beta antibody (ab5792)
Key features and details
- Rabbit polyclonal to Retinoic Acid Receptor beta
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Retinoic Acid Receptor beta antibody
See all Retinoic Acid Receptor beta primary antibodies -
Description
Rabbit polyclonal to Retinoic Acid Receptor beta -
Host species
Rabbit -
Specificity
This antibody shows slight cross-reactivity to RAR alpha but does not detect RAR gamma. -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to Mouse Retinoic Acid Receptor beta aa 429-448.
Sequence:PSUSPSSVENSGVSQSPLLQ
(Peptide available asab5897) -
Positive control
- WB: SH-SY5Y cell extract ; HEK-293 ICC/IF: SH-SY5Y
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab5792 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/100.
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WB |
1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 53 kDa).
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Notes |
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ICC/IF
1/100. |
WB
1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 53 kDa). |
Target
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Function
Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence or presence of hormone ligand, acts mainly as an activator of gene expression due to weak binding to corepressors. In concert with RARG, required for skeletal growth, matrix homeostasis and growth plate function. -
Involvement in disease
Microphthalmia, syndromic, 12 -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Domain
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. -
Cellular localization
Cytoplasm and Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5915 Human
- Entrez Gene: 218772 Mouse
- Omim: 180220 Human
- SwissProt: P10826 Human
- SwissProt: P22605 Mouse
- Unigene: 543218 Human
- Unigene: 581530 Human
- Unigene: 654490 Human
see all -
Alternative names
- HAP antibody
- HBV-activated protein antibody
- NR1B2 antibody
see all
Images
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All lanes : Anti-Retinoic Acid Receptor beta antibody (ab5792) at 1 µg/ml
Lane 1 : SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate
Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
Lane 3 : PANC-1 (Human pancreatic epithelial cancinoma cell line) whole cell lysate
Lane 4 : OVCAR-3 (Human ovary adenocarcinoma cell line) whole cell lysate
Lane 5 : BeWo (human placenta choriocarcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP at 1/4000 dilution
Predicted band size: 53 kDa
Additional bands at: ~58.50 kDa. We are unsure as to the identity of these extra bands.Detection: chemiluminescence
Western blot demonstrating antibody specificity by detection of differential basal expression of the target across cell lines owing to their inherent genetic constitution. The expression was observed in SH-SY5Y and HEK-293 and not seen in PANC-1, OVCAR-3 and BeWo using ab5792.
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All lanes : Anti-Retinoic Acid Receptor beta antibody (ab5792) at 1/1000 dilution
Lane 1 : SH-SY5Y cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : Mouse heart cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 53 kDa
Observed band size: 58 kDa why is the actual band size different from the predicted? -
Immunofluorescent analysis of SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate cells on 70% confluent log phase labeling Retinoic Acid Receptor beta. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with ab5792 at 1/100 dilution in 0.1% BSA and incubated overnight at 4°C and then labeled with Goat anti-Rabbit IgG (H+L) secondary antibody, Alexa Fluor® 488 conjugate at 1/2000 dilution for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (1/300 dilution). Panel d is a merged image showing nuclear and cytoplasmic localization. Panel e represents BeWo (human placenta choriocarcinoma cell line) having no expression of Retinoic Acid Receptor beta. The images were captured at 60X magnification.
Datasheets and documents
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Datasheet download
References (0)
ab5792 has not yet been referenced specifically in any publications.