Anti-Retinoid X Receptor alpha/RXRA antibody (ab24363)
Key features and details
- Goat polyclonal to Retinoid X Receptor alpha/RXRA
- Suitable for: Flow Cyt (Intra), WB, ICC
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Retinoid X Receptor alpha/RXRA antibody
See all Retinoid X Receptor alpha/RXRA primary antibodies -
Description
Goat polyclonal to Retinoid X Receptor alpha/RXRA -
Host species
Goat -
Specificity
This does not cross-react with either RXR beta or gamma. -
Tested applications
Suitable for: Flow Cyt (Intra), WB, ICCmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide corresponding to Human Retinoid X Receptor alpha/RXRA aa 14-28 (N terminal).
Sequence:(C)QVNSSLTSPTGRGSM
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Positive control
- Flow Cyt (Intra): MCF7 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab24363 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
Use a concentration of 10 µg/ml.
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WB | (1) |
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 51 kDa).
1 hour primary incubation is recommended for this product. |
ICC |
Use a concentration of 10 µg/ml.
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Notes |
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Flow Cyt (Intra)
Use a concentration of 10 µg/ml. |
WB
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 51 kDa). 1 hour primary incubation is recommended for this product. |
ICC
Use a concentration of 10 µg/ml. |
Target
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Function
Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. The high affinity ligand for RXRs is 9-cis retinoic acid. RXRA serves as a common heterodimeric partner for a number of nuclear receptors. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone acetylation, chromatin condensation and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. The RXRA/PPARA heterodimer is required for PPARA transcriptional activity on fatty acid oxidation genes such as ACOX1 and the P450 system genes. -
Tissue specificity
Highly expressed in liver, also found in lung, kidney and heart. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR2 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Domain
Composed of three domains: a modulating N-terminal domain (AF1 domain), a DNA-binding domain and a C-terminal ligand-binding domain (AF2 domain). -
Post-translational
modificationsPhosphorylated on serine and threonine residues mainly in the N-terminal modulating domain. Constiutively phosphorylated on Ser-21 in the presence or absence of ligand. Under stress conditions, hyperphosphorylated by activated JNK on Ser-56, Ser-70, Thr-82 and Ser-260 (By similarity). Phosphorylated on Ser-27, in vitro, by PKA. This phosphorylation is required for repression of cAMP-mediated transcriptional activity of RARA.
Sumoylation negatively regulates transcriptional activity. Desumoylated specifically by SENP6. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 6256 Human
- Entrez Gene: 20181 Mouse
- Entrez Gene: 25271 Rat
- Omim: 180245 Human
- SwissProt: P19793 Human
- SwissProt: P28700 Mouse
- SwissProt: Q05343 Rat
- Unigene: 590886 Human
see all -
Alternative names
- FLJ00280 antibody
- FLJ00318 antibody
- FLJ16020 antibody
see all
Images
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All lanes : Anti-Retinoid X Receptor alpha/RXRA antibody (ab24363) at 0.3 µg/ml
Lane 1 : HeLa nuclear lysate (in RIPA buffer)
Lane 2 : HeLa nuclear lysate (in RIPA buffer) with peptide
Lane 3 : K562 nuclear lysate (in RIPA buffer)
Lane 4 : K562 nuclear lysate (in RIPA buffer) with peptide
Lysates/proteins at 35 µg per lane.
Predicted band size: 51 kDa
Observed band size: 55-60 kDa why is the actual band size different from the predicted?Primary incubation was 1 hour. Detected by chemiluminescence.
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Immunocytochemistry analysis of MCF7 cells labelling Retinoid X Receptor alpha/RXRA with ab24363 at 10 µg/mL showing strong nuclear staining. Cells were fixed with paraformaldehyde and permeabilized with 0.15% Triton. Primary incubation for 1 hour. Alexa Fluor® 488 secondary antibody at 2 µg/mL (green). Actin filaments were stained with phalloidin (red). Nuclear DNA was labelled with DAPI (blue).
Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor® 488 secondary antibody (2 µg/mL).
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Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line) labelling Retinoid X Receptor alpha/RXRA with ab24363. Cells permeabilized with 0.5% Triton. Primary incubation 1 hour (10 µg/mL) followed by Alexa Fluor® 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor® 488 secondary antibody.
Datasheets and documents
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Datasheet download
References (3)
ab24363 has been referenced in 3 publications.
- Zhao X et al. Risk-Associated Long Noncoding RNA FOXD3-AS1 Inhibits Neuroblastoma Progression by Repressing PARP1-Mediated Activation of CTCF. Mol Ther 26:755-773 (2018). PubMed: 29398485
- Castella B et al. The ATP-binding cassette transporter A1 regulates phosphoantigen release and V?9Vd2 T cell activation by dendritic cells. Nat Commun 8:15663 (2017). PubMed: 28580927
- Nickkho-Amiry M et al. Peroxisome proliferator-activated receptors modulate proliferation and angiogenesis in human endometrial carcinoma. Mol Cancer Res 10:441-53 (2012). WB, IHC-P ; Human . PubMed: 22205725