Anti-Retinoid X Receptor alpha antibody (ab80453)

Overview

  • Product nameAnti-Retinoid X Receptor alpha antibodySee all Retinoid X Receptor alpha primary antibodies ...
  • Description
    Rabbit polyclonal to Retinoid X Receptor alpha
  • Tested applicationsELISA, WB more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Guinea pig, Cow
  • Immunogen

    Synthetic peptide: SLGPGIGSPG QLHSPISTLS SPINGMGPPF SVISSPMGPH SMSVPTTPTL , corresponding to N terminal amino acids 35-84 of Human Retinoid X Receptor alpha (NP_002948)

  • Positive control
    • Transfected 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab80453 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

WB Use a concentration of 1 µg/ml. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionReceptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. The high affinity ligand for RXRs is 9-cis retinoic acid. RXRA serves as a common heterodimeric partner for a number of nuclear receptors. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone acetylation, chromatin condensation and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. The RXRA/PPARA heterodimer is required for PPARA transcriptional activity on fatty acid oxidation genes such as ACOX1 and the P450 system genes.
  • Tissue specificityHighly expressed in liver, also found in lung, kidney and heart.
  • Sequence similaritiesBelongs to the nuclear hormone receptor family. NR2 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • DomainComposed of three domains: a modulating N-terminal domain (AF1 domain), a DNA-binding domain and a C-terminal ligand-binding domain (AF2 domain).
  • Post-translational
    modifications
    Phosphorylated on serine and threonine residues mainly in the N-terminal modulating domain. Constiutively phosphorylated on Ser-21 in the presence or absence of ligand. Under stress conditions, hyperphosphorylated by activated JNK on Ser-56, Ser-70, Thr-82 and Ser-260 (By similarity). Phosphorylated on Ser-27, in vitro, by PKA. This phosphorylation is required for repression of cAMP-mediated transcriptional activity of RARA.
    Sumoylation negatively regulates transcriptional activity. Desumoylated specifically by SENP6.
  • Cellular localizationNucleus.
  • Target information above from: UniProt accession P19793 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • FLJ00280 antibody
    • FLJ00318 antibody
    • FLJ16020 antibody
    • FLJ16733 antibody
    • MGC102720 antibody
    • NR2B1 antibody
    • Nuclear receptor subfamily 2 group B member 1 antibody
    • OTTHUMP00000022510 antibody
    • Retinoic acid receptor RXR alpha antibody
    • Retinoic acid receptor RXR-alpha antibody
    • Retinoid X nuclear receptor alpha antibody
    • Retinoid X receptor alpha antibody
    • RXR alpha1 antibody
    • Rxra antibody
    • RXRA_HUMAN antibody
    • RXRalpha1 antibody
    see all

Anti-Retinoid X Receptor alpha antibody images

  • Anti-Retinoid X Receptor alpha antibody (ab80453) at 1 µg/ml + Transfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 51 kDa
    Observed band size : 51 kDa
    Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.

References for Anti-Retinoid X Receptor alpha antibody (ab80453)

ab80453 has not yet been referenced specifically in any publications.

Product Wall

I am sorry without purchase order number nothing can be done in this case. OK! Could you tell me from where you bought this antibody; if you have bought this from one of our Chinese distributor, then you can contact them for purchase order number; they...

Read More

I am sorry I am not able to find the reason why the antibody is not working. Could you let us know how you would like to proceed in this case? Do you want free of charge replacement or refund.

Please be also advised that we cannot process yo...

Read More

Thank you very much for filling the feedback form. Your email has been assigned to me.

I have checked our previous conversations and can say that I have been waiting for answers to my questions since December 16th 2011. I am now following up ...

Read More

Thank you for contacting us. This lot has been in stock for a while now and we have not received any complaint other than this. We would like to have more information so that we can investigate the case further; - Could you provide the order numb...

Read More

Thank you for your enquiry regarding ab80453 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody. Additionally, thank you for supplying an image, as this has...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"