Anti-Retinoid X Receptor alpha antibody (ab86027)

Overview

• Product nameAnti-Retinoid X Receptor alpha antibodySee all Retinoid X Receptor alpha primary antibodies ...
• Description
  Rabbit polyclonal to Retinoid X Receptor alpha
• Tested applicationsWB more details
• Species reactivity
  Reacts with: Mouse, Human
  
• Immunogen

  Three synthetic peptides (human) conjugated to KLH

• Positive controlAn induced culture of E. coli

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
  Constituents: Whole serum
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Lipid and lipoprotein metabolism
  • Lipid metabolism

  • Cancer
  • Signal transduction
  • Nuclear signaling
  • Nuclear hormone receptors
  • Retanoic and retanoid

  • Cancer
  • Cancer Metabolism
  • Metabolic signaling pathway
  • Metabolism of lipids and lipoproteins

  • Signal Transduction
  • Signaling Pathway
  • Nuclear Signaling
  • Nuclear Hormone Receptors
  • Retinoic & Retinoid

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Domain Families
  • Zinc Finger

  • Epigenetics and Nuclear Signaling
  • Nuclear Signaling Pathways
  • Nuclear Receptors
  • Retinoic & Retinoid

Applications

Target

• FunctionReceptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. The high affinity ligand for RXRs is 9-cis retinoic acid. RXRA serves as a common heterodimeric partner for a number of nuclear receptors. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone acetylation, chromatin condensation and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. The RXRA/PPARA heterodimer is required for PPARA transcriptional activity on fatty acid oxidation genes such as ACOX1 and the P450 system genes.
• Tissue specificityHighly expressed in liver, also found in lung, kidney and heart.
• Sequence similaritiesBelongs to the nuclear hormone receptor family. NR2 subfamily.
  Contains 1 nuclear receptor DNA-binding domain.
• DomainComposed of three domains: a modulating N-terminal domain (AF1 domain), a DNA-binding domain and a C-terminal ligand-binding domain (AF2 domain).
• Post-translational
  modificationsPhosphorylated on serine and threonine residues mainly in the N-terminal modulating domain. Constiutively phosphorylated on Ser-21 in the presence or absence of ligand. Under stress conditions, hyperphosphorylated by activated JNK on Ser-56, Ser-70, Thr-82 and Ser-260 (By similarity). Phosphorylated on Ser-27, in vitro, by PKA. This phosphorylation is required for repression of cAMP-mediated transcriptional activity of RARA.
  Sumoylation negatively regulates transcriptional activity. Desumoylated specifically by SENP6.
• Cellular localizationNucleus.

Target information above from: UniProt accession P19793 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 6256 Human
  • Entrez Gene: 20181 Mouse
  • Omim: 180245 Human
  • SwissProt: P19793 Human
  • SwissProt: P28700 Mouse
  • Unigene: 590886 Human
  • Unigene: 24624 Mouse

• Alternative names
  FLJ00280 antibodyFLJ00318 antibodyFLJ16020 antibody

  FLJ16733 antibodyMGC102720 antibodyNR2B1 antibodyNuclear receptor subfamily 2 group B member 1 antibodyOTTHUMP00000022510 antibodyRetinoic acid receptor RXR alpha antibodyRetinoic acid receptor RXR-alpha antibodyRetinoid X nuclear receptor alpha antibodyRetinoid X receptor alpha antibodyRXR alpha1 antibodyRxra antibodyRXRA_HUMAN antibodyRXRalpha1 antibody

  see all

Anti-Retinoid X Receptor alpha antibody images

• 

  Western blot - Retinoid X Receptor alpha antibody (ab86027)
  All lanes : Anti-Retinoid X Receptor alpha antibody (ab86027) at 1/1000 dilution
  
  Lane 1 : An un-induced culture of E. coli
  Lane 2 : An induced culture of E. coli
  
  Lysates/proteins at 1/5000 dilution per lane.
  
  Secondary
  Anti-Rabbit IgG HRP at 1/1000 dilution
  
  Predicted band size : 51 kDa
  Observed band size : 65 kDa (why is the actual band size different from the predicted?)
  Additional bands at : 45 kDa,46 kDa. We are unsure as to the identity of these extra bands.
  
  Exposure time : 60 minutes