Anti-Retinoid X Receptor alpha antibody (ab86027)

Overview

  • Product nameAnti-Retinoid X Receptor alpha antibodySee all Retinoid X Receptor alpha primary antibodies ...
  • Description
    Rabbit polyclonal to Retinoid X Receptor alpha
  • Tested applicationsWBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Three synthetic peptides (human) conjugated to KLH

  • Positive control
    • An induced culture of E. coli

Applications

Our Abpromise guarantee covers the use of ab86027 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: 1/1000. Detects a band of approximately 65 kDa (predicted molecular weight: 51 kDa). Tag is 14kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionReceptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. The high affinity ligand for RXRs is 9-cis retinoic acid. RXRA serves as a common heterodimeric partner for a number of nuclear receptors. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone acetylation, chromatin condensation and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. The RXRA/PPARA heterodimer is required for PPARA transcriptional activity on fatty acid oxidation genes such as ACOX1 and the P450 system genes.
    • Tissue specificityHighly expressed in liver, also found in lung, kidney and heart.
    • Sequence similaritiesBelongs to the nuclear hormone receptor family. NR2 subfamily.
      Contains 1 nuclear receptor DNA-binding domain.
    • DomainComposed of three domains: a modulating N-terminal domain (AF1 domain), a DNA-binding domain and a C-terminal ligand-binding domain (AF2 domain).
    • Post-translational
      modifications
      Phosphorylated on serine and threonine residues mainly in the N-terminal modulating domain. Constiutively phosphorylated on Ser-21 in the presence or absence of ligand. Under stress conditions, hyperphosphorylated by activated JNK on Ser-56, Ser-70, Thr-82 and Ser-260 (By similarity). Phosphorylated on Ser-27, in vitro, by PKA. This phosphorylation is required for repression of cAMP-mediated transcriptional activity of RARA.
      Sumoylation negatively regulates transcriptional activity. Desumoylated specifically by SENP6.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • FLJ00280 antibody
      • FLJ00318 antibody
      • FLJ16020 antibody
      • FLJ16733 antibody
      • MGC102720 antibody
      • NR2B1 antibody
      • Nuclear receptor subfamily 2 group B member 1 antibody
      • OTTHUMP00000022510 antibody
      • Retinoic acid receptor RXR alpha antibody
      • Retinoic acid receptor RXR-alpha antibody
      • Retinoid X nuclear receptor alpha antibody
      • Retinoid X receptor alpha antibody
      • RXR alpha1 antibody
      • Rxra antibody
      • RXRA_HUMAN antibody
      • RXRalpha1 antibody
      see all

    Anti-Retinoid X Receptor alpha antibody images

    • All lanes : Anti-Retinoid X Receptor alpha antibody (ab86027) at 1/1000 dilution

      Lane 1 : An un-induced culture of E. coli
      Lane 2 : An induced culture of E. coli

      Lysates/proteins at 1/5000 dilution per lane.

      Secondary
      Anti-Rabbit IgG HRP at 1/1000 dilution

      Predicted band size : 51 kDa
      Observed band size : 65 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 45 kDa,46 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 60 minutes

    References for Anti-Retinoid X Receptor alpha antibody (ab86027)

    ab86027 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab86027.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"