Anti-Retinoid X Receptor beta/RXRB antibody (ab221115)
Key features and details
- Rabbit polyclonal to Retinoid X Receptor beta/RXRB
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Retinoid X Receptor beta/RXRB antibody
See all Retinoid X Receptor beta/RXRB primary antibodies -
Description
Rabbit polyclonal to Retinoid X Receptor beta/RXRB -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Recombinant fragment corresponding to Human Retinoid X Receptor beta/RXRB aa 50-150.
Database link: P28702 -
Positive control
- RT-4 cell lysates; Human tonsil lysate; SK-MEL-30 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab221115 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
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WB |
1/100 - 1/250. Predicted molecular weight: 57 kDa.
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Notes |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
WB
1/100 - 1/250. Predicted molecular weight: 57 kDa. |
Target
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Function
Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE). -
Tissue specificity
Expressed in a variety of tumor cell lines. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR2 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Domain
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 6257 Human
- Entrez Gene: 20182 Mouse
- Omim: 180246 Human
- SwissProt: P28702 Human
- SwissProt: P28704 Mouse
- Unigene: 388034 Human
- Unigene: 1243 Mouse
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Alternative names
- DAUD I6 antibody
- DAUDI6 antibody
- H 2RIIBP antibody
see all
Images
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Immunofluorescent analysis of PFA-fixed, Triton X-100 permeabilized SK-MEL-30 cells labeling Retinoid X Receptor beta/RXRB with ab221115 at 4 µg/ml (green).
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All lanes : Anti-Retinoid X Receptor beta/RXRB antibody (ab221115) at 1/100 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human plasma
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Predicted band size: 57 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab221115 has been referenced in 1 publication.
- Zeng Y et al. Overexpression of retinoid X receptor beta provides protection against oxidized low-density lipoprotein-induced inflammation via regulating PGC1a-dependent mitochondrial homeostasis in endothelial cells. Biochem Pharmacol 188:114559 (2021). PubMed: 33872571