Anti-Rffl antibody (ab47994)

Overview

• Product nameAnti-Rffl antibodySee all Rffl primary antibodies ...
• Description
  Rabbit polyclonal to Rffl
• SpecificityThis antibody recognizes RFFL (41kDa). Reactivity with isoforms 2 (39.7kDa) and 3 (36.6kDa) should also be expected.
• Tested applicationsELISA, WB, IHC-P more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse, Rat, Cow, Dog
• Immunogen

  Recombinant protein corresponding to amino acids 1-363 of human RFFL protein.

• Positive controlWB: 293T cell lysate

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.01% Sodium Azide
  Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
• Concentration information loading...
• PurityProtein A purified
• Purification notesFollowing chromatography, the product was transferred to the storage buffer via exhaustive dialysis. The product was sterile filtered.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Signal Transduction
  • Signaling Pathway
  • Nuclear Signaling
  • NFkB Pathway

  • Epigenetics and Nuclear Signaling
  • Ubiquitin & Ubiquitin Like Modifiers
  • E3 Ubiquitin Ligases

  • Epigenetics and Nuclear Signaling
  • Nuclear Signaling Pathways
  • NFkB pathway

  • Cell Biology
  • Apoptosis
  • Intracellular
  • p53 Pathway

  • Cell Biology
  • Apoptosis
  • Intracellular
  • Survivin / IAPs

  • Cell Biology
  • Apoptosis
  • Intracellular
  • Caspases etc
  • Inhibitors

  • Cell Biology
  • Proteolysis / Ubiquitin
  • Proteasome / Ubiquitin
  • Ubiquitin E2s and E3s
  • Other E3 Ligases

Applications

Target

• FunctionHas E3 ubiquitin protein ligase activity. Regulates the levels of CASP8 and CASP10 by targeting them for proteasomal degradation. Has anti-apoptotic activity. May bind phosphatidylinositol phosphates.
• Tissue specificityUbiquitous. Detected in spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes.
• PathwayProtein modification; protein ubiquitination.
• Sequence similaritiesContains 1 FYVE-type zinc finger.
  Contains 1 RING-type zinc finger.
  Contains 2 SAP domains.
• Post-translational
  modificationsRapidly degraded after stimulation with TNFSF10, probably by caspases.
  Auto-ubiquitinated (in vitro).
  Palmitoylated.
• Cellular localizationCytoplasm. Membrane.

Target information above from: UniProt accession Q8WZ73 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 530263 Cow
  • Entrez Gene: 117584 Human
  • Entrez Gene: 67338 Mouse
  • Entrez Gene: 282844 Rat
  • Omim: 609735 Human
  • SwissProt: Q8WZ73 Human
  • SwissProt: Q6ZQM0 Mouse
  • SwissProt: Q8CIN9 Rat

  • Unigene: 13680 Human
  • Unigene: 341608 Mouse
  • Unigene: 159718 Rat

  see all

• Alternative names
  CARP-2 antibodyCARP2 antibodyCaspase regulator CARP2 antibody

  Caspases 8 and 10 associated RING finger protein 2 antibodycaspases-8 and -10- associated RING finger protein 2 antibodyCaspases-8 and -10-associated RING finger protein 2 antibodyE3 ubiquitin protein ligase rififylin antibodyE3 ubiquitin-protein ligase rififylin antibodyFring antibodyFYVE RING finger protein Sakura antibodyFYVE-RING finger protein Sakura antibodyRffl antibodyRFFL_HUMAN antibodyRififylin antibodyRING finger and FYVE like domain containing protein antibodyRING finger and FYVE-like domain-containing protein 1 antibodyRING finger protein 189 antibodyRING finger protein 34 like antibodyRING finger protein 34-like antibodyRNF189 antibodyRNF34L antibody

  see all

Anti-Rffl antibody images

• 

  Western blot - Rffl antibody (ab47994)
  All lanes : Anti-Rffl antibody (ab47994) at 1/1000 dilution
  
  Lane 1 : Empty vector 293T cell lysate with 5% BLOTTO, 0.1% Tween 20 in PBS
  Lane 2 : RNF34 transfected lysate with 5% BLOTTO, 0.1% Tween 20 in PBS
  Lane 3 : RFFL transfected lysate with 5% BLOTTO, 0.1% Tween 20 in PBS
  
  Lysates/proteins at 20 µl per lane.
  
  
  Predicted band size : 41 kDa
  Observed band size : 41 kDa
  Additional bands at : 58 kDa. We are unsure as to the identity of these extra bands.