Overview

  • Product nameAnti-Rffl antibodySee all Rffl primary antibodies ...
  • Description
    Rabbit polyclonal to Rffl
  • SpecificityThis antibody recognizes RFFL (41kDa). Reactivity with isoforms 2 (39.7kDa) and 3 (36.6kDa) should also be expected.
  • Tested applicationsELISA, WB, IHC-P more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog
  • Immunogen

    Recombinant protein corresponding to amino acids 1-363 of human RFFL protein.

  • Positive control
    • WB: 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab47994 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ELISA 1/4000 - 1/20000.
WB 1/500 - 1/3000. Predicted molecular weight: 41 kDa.
IHC-P 1/500 - 1/3000.

Target

  • FunctionHas E3 ubiquitin protein ligase activity. Regulates the levels of CASP8 and CASP10 by targeting them for proteasomal degradation. Has anti-apoptotic activity. May bind phosphatidylinositol phosphates.
  • Tissue specificityUbiquitous. Detected in spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesContains 1 FYVE-type zinc finger.
    Contains 1 RING-type zinc finger.
    Contains 2 SAP domains.
  • Post-translational
    modifications
    Rapidly degraded after stimulation with TNFSF10, probably by caspases.
    Auto-ubiquitinated (in vitro).
    Palmitoylated.
  • Cellular localizationCytoplasm. Membrane.
  • Target information above from: UniProt accession Q8WZ73 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • CARP-2 antibody
    • CARP2 antibody
    • Caspase regulator CARP2 antibody
    • Caspases 8 and 10 associated RING finger protein 2 antibody
    • caspases-8 and -10- associated RING finger protein 2 antibody
    • Caspases-8 and -10-associated RING finger protein 2 antibody
    • E3 ubiquitin protein ligase rififylin antibody
    • E3 ubiquitin-protein ligase rififylin antibody
    • Fring antibody
    • FYVE RING finger protein Sakura antibody
    • FYVE-RING finger protein Sakura antibody
    • Rffl antibody
    • RFFL_HUMAN antibody
    • Rififylin antibody
    • RING finger and FYVE like domain containing protein antibody
    • RING finger and FYVE-like domain-containing protein 1 antibody
    • RING finger protein 189 antibody
    • RING finger protein 34 like antibody
    • RING finger protein 34-like antibody
    • RNF189 antibody
    • RNF34L antibody
    see all

Anti-Rffl antibody images

  • All lanes : Anti-Rffl antibody (ab47994) at 1/1000 dilution

    Lane 1 : Empty vector 293T cell lysate with 5% BLOTTO, 0.1% Tween 20 in PBS
    Lane 2 : RNF34 transfected lysate with 5% BLOTTO, 0.1% Tween 20 in PBS
    Lane 3 : RFFL transfected lysate with 5% BLOTTO, 0.1% Tween 20 in PBS

    Lysates/proteins at 20 µl per lane.


    Predicted band size : 41 kDa
    Observed band size : 41 kDa
    Additional bands at : 58 kDa. We are unsure as to the identity of these extra bands.

References for Anti-Rffl antibody (ab47994)

ab47994 has not yet been referenced specifically in any publications.

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