![Western blot - Rho antibody [EP487Y] (ab40673) image](#ab40673.jpg)
![Immunohistochemistry (Paraffin-embedded sections) - Rho antibody [EP487Y] (ab40673) image](#ab40673_1.jpg)
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Product name
Anti-Rho antibody [EP487Y]
See all Rho products (3) ...
Description
Rabbit monoclonal [EP487Y] to Rho
Specificity
This antibody is specific for Rho. It is also expected to detect RhoA, RhoB and RhoC.
Tested applications
WB, IP, ICC, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide corresponding to residues near the N terminus of Human Rho.
Positive control
HL60 cell lysate.
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EP487Y
Isotype
IgG
Research areas
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
Applications
Show applications key
Our Abpromise guarantee covers the use of ab40673 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP
ICC
- 1 Image
IHC-P
Application notes
ICC: 1/250 - 1/500.
IHC-P: 1/100 - 1/250.
IP: 1/50.
WB: 1/2000 - 1/10000. Detects a band of approximately 22 kDa (predicted molecular weight: 21 kDa).
Is unsuitable for FACS.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
Rho proteins belong to the Ras super family of low molecular weight GTPases, which includes RAS, RHO, RAB, RAN, RAD and ARF. These small GTP binding proteins function as molecular switches that regulate a multitude of biological processes including cell proliferation, apoptosis, differentiation, cytoskeletal reorganisation, and membrane trafficking.
Cellular localization
Membrane; lipid-anchor.
Alternative names
- Aplysia ras related homolog 12 antibody
- ARH12 antibody
- ARHA antibody
see all
Database links
- Entrez Gene: 387 Human
- Entrez Gene: 388 Human
- Entrez Gene: 389 Human
- Entrez Gene: 11848 Mouse
- Entrez Gene: 117273 Rat
- SwissProt: P61586 Human
- SwissProt: P62745 Human
- SwissProt: P08134 Human
see all
Anti-Rho antibody [EP487Y] images:
Western blot - Rho antibody [EP487Y] (ab40673)
![Western blot - Rho antibody [EP487Y] (ab40673)](/ps/datasheet/Images/40/ab40673/ab40673.jpg)
Anti-Rho antibody [EP487Y] (ab40673) + 10ug HL60 cell lysate
Predicted band size : 21 kDa
Observed band size : 22 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Paraffin-embedded sections) - Rho antibody [EP487Y] (ab40673)
![Immunohistochemistry (Paraffin-embedded sections) - Rho antibody [EP487Y] (ab40673)](/ps/datasheet/Images/40/ab40673/ab40673_1.jpg)
ab40673 at a 1:100 dilution staining Rho in human prostate carcinoma tissue.
References for Anti-Rho antibody [EP487Y] (ab40673)
ab40673 has not yet been referenced specifically in any publications.
Publishing research using ab40673? Please let us know so that we can cite the reference in this datasheet
![Rho antibody [EP487Y] for WB in Mouse (40673)](/ps/Reviews/Images/ab10352_53322.jpg)
![Rho antibody [EP487Y] for Western blot in Caenorhabditis elegans (40673)](/ps/datasheet/images/40/ab40673/Rho-Primary-antibodies-ab40673-4.JPG)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"