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Anti-Rho antibody [Y486] (ab32046)

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Reassurance, Refunds & Replacements

If your product does not perform as described on this datasheet, we will refund or replace your product...

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Overview

Product name

Anti-Rho antibody [Y486]
See all Rho products (3) ...

Description

Rabbit monoclonal [Y486] to Rho

Specificity

The antibody is predicted to detect RhoA, RhoB and RhoC based on sequence analysis.

Tested applications

WB, IHC-P, ICC/IFmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

A synthetic peptide corresponding to residues in the N-term of human Rho.

Positive control

PC12 cell lysate and hepatocellular carcinoma.

General notes

Produced under U.S. Patent No. 5,675,063.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%

Concentration

Concentration information loading...

Purity

IgG fraction

Clonality

Monoclonal

Clone number

Y486

Isotype

IgG

Research areas

Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family

  • Western blot - Rho antibody [Y486] (ab32046)Western blot - Rho antibody [Y486] (ab32046) image (enlarge)

  • Immunohistochemistry (Paraffin-embedded sections) - Rho antibody [Y486] (ab32046)Immunohistochemistry (Paraffin-embedded sections) - Rho antibody [Y486] (ab32046) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab32046 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ICC/IF: 1/100 - 1/250.
IHC-P: 1/50 - 1/100.

WB: 1/2000. Detects a band of approximately 22 kDa (predicted molecular weight: 21 kDa).

Is unsuitable for FACS or IP.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

Rho proteins belong to the Ras super family of low molecular weight GTPases, which includes RAS, RHO, RAB, RAN, RAD and ARF. These small GTP binding proteins function as molecular switches that regulate a multitude of biological processes including cell proliferation, apoptosis, differentiation, cytoskeletal reorganisation, and membrane trafficking.

Cellular localization

Membrane; lipid-anchor.

Alternative names

  • Aplysia ras related homolog 12 antibody
  • ARH12 antibody
  • ARHA antibody
  • H12 antibody
  • Oncogene RHO H12 antibody
  • ras homolog gene family antibody
  • ras homolog gene family member A antibody
  • ras homolog gene family member B antibody
  • ras homolog gene family member C antibody
  • RHO12 antibody
  • rhoa antibody
  • rhob antibody
  • rhoc antibody
  • rhod antibody
  • RHOH12 antibody
  • Small GTP binding protein RhoA antibody
  • Transforming protein RhoA antibody
see all

Database links

see all

Anti-Rho antibody [Y486] images:

  Western blot - Rho antibody [Y486] (ab32046)

Western blot - Rho antibody [Y486] (ab32046)

Anti-Rho antibody [Y486] (ab32046) at 1/2000 dilution + PC12 cell lysate

Predicted band size : 21 kDa
Observed band size : 22 kDa (why is the actual band size different from the predicted?)

  Immunohistochemistry (Paraffin-embedded sections) - Rho antibody [Y486] (ab32046)

Immunohistochemistry (Paraffin-embedded sections) - Rho antibody [Y486] (ab32046)

Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma using ab32046 at 1/100 dilution.

References for Anti-Rho antibody [Y486] (ab32046)

This product has been referenced in:

  • Ruggeri RM  et al. The tyrosine kinase receptor c-met, its cognate ligand HGF and the tyrosine kinase receptor trasducers STAT3, PI3K and RHO in thyroid nodules associated with Hashimoto's thyroiditis: an immunohistochemical characterization. Eur J Histochem 54:e24 (2010).Read more (PubMed: 20558345) »
  • Laumanns IP  et al. The noncanonical WNT pathway is operative in idiopathic pulmonary arterial hypertension. Am J Respir Cell Mol Biol 40:683-91 (2009).Read more (PubMed: 19029018) »

See all 2 publications for this product

Publishing research using ab32046? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"