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Product name
Anti-RhoA antibody
See all RhoA products (11) ...
Description
Mouse monoclonal to RhoA
Tested applications
WB, IHC-P, Flow Cyt, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant full length protein, corresponding to amino acids 1-194 of Human RhoA
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Isotype
IgG1
Light chain type
lambda
Research areas
Cancer >> Signal transduction >> G protein signaling >> Small G proteins >> Ras family
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Assembly
Applications
Show applications key
Our Abpromise guarantee covers the use of ab54835 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 22 kDa.
IHC-P: Use a concentration of 5 µg/ml.
Flow Cyt: Use 1µg for 106 cells.
ICC/IF: Use a concentration of 10 µg/ml.
Target
Function
Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. May be an activator of PLCE1. Activated by ARHGEF2, which promotes the exchange of GDP for GTP.
Sequence similarities
Belongs to the small GTPase superfamily. Rho family.
Domain
The basic-rich region is essential for yopT recognition and cleavage.
Post-translational
modifications
Substrate for botulinum ADP-ribosyltransferase.
Cleaved by yopT protease when the cell is infected by some Yersinia pathogens. This removes the lipid attachment, and leads to its displacement from plasma membrane and to subsequent cytoskeleton cleavage.
AMPylation at Tyr-34 and Thr-37 are mediated by bacterial enzymes in case of infection by H.somnus and V.parahaemolyticus, respectively. AMPylation occurs in the effector region and leads to inactivation of the GTPase activity by preventing the interaction with downstream effectors, thereby inhibiting actin assembly in infected cells. It is unclear whether some human enzyme mediates AMPylation; FICD has such ability in vitro but additional experiments remain to be done to confirm results in vivo.
Ubiquitinated by the BCR(BACURD1) and BCR(BACURD2) E3 ubiquitin ligase complexes, leading to its degradation by the proteasome, thereby regulating the actin cytoskeleton and cell migration.
Cellular localization
Cell membrane. Cytoplasm > cytoskeleton.
Target information above from: UniProt accessionP61586
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Aplysia ras related homolog 12 antibody
- ARH 12 antibody
- ARH12 antibody
see all
Database links
- Entrez Gene: 387 Human
- Omim: 165390 Human
- SwissProt: P61586 Human
- Unigene: 247077 Human
Anti-RhoA antibody images:
IHC-P - RhoA antibody (ab54835)
RhoA antibody (ab54835) used in immunohistochemistry at 5ug/ml on formalin fixed and paraffin embedded human lymphoma tissue.
Western blot - RhoA antibody (ab54835)
RhoA antibody (ab54835) at 1ug/lane + HL-60 cell lysate at 25ug/lane.
Immunocytochemistry/ Immunofluorescence - RhoA antibody (ab54835)
ab54835 at 10 ug/ml staining RhoA in human Hela cells by Immunocytochemistry / Immunofluorescence.
Immunocytochemistry/ Immunofluorescence - RhoA antibody (ab54835)
ICC/IF image of ab54835 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab54835, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Flow Cytometry-RhoA antibody(ab54835)
Overlay histogram showing HeLa cells stained with ab54835 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54835, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
References for Anti-RhoA antibody (ab54835)
This product has been referenced in:
- Gou L et al. Proteomic identification of RhoA as a potential biomarker for proliferation and metastasis in hepatocellular carcinoma. J Mol Med : (2011). IHC.Read more (PubMed: 21475975) »
- Valastyan S et al. Concurrent suppression of integrin alpha5, radixin, and RhoA phenocopies the effects of miR-31 on metastasis. Cancer Res 70:5147-54 (2010). WB; Human.Read more (PubMed: 20530680) »
See all 5 publications for this product
Publishing research using ab54835? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"