Recombinant Anti-RhoGDI antibody [EPR3772] (ab108977)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3772] to RhoGDI
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-RhoGDI antibody [EPR3772]
See all RhoGDI primary antibodies -
Description
Rabbit monoclonal [EPR3772] to RhoGDI -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK293T, Jurkat, HeLa, HL-60 and NIH/3T3 cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3772 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab108977 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Detects a band of approximately 26 kDa (predicted molecular weight: 23 kDa).
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Notes |
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WB
1/1000 - 1/5000. Detects a band of approximately 26 kDa (predicted molecular weight: 23 kDa). |
Target
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Function
Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. -
Sequence similarities
Belongs to the Rho GDI family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 396 Human
- Entrez Gene: 192662 Mouse
- Entrez Gene: 360678 Rat
- Omim: 601925 Human
- SwissProt: P52565 Human
- SwissProt: Q99PT1 Mouse
- SwissProt: Q5XI73 Rat
- Unigene: 159161 Human
see all -
Alternative names
- ARHGDIA antibody
- fa96g11 antibody
- GDIA 1 antibody
see all
Images
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All lanes : Anti-RhoGDI antibody [EPR3773] (ab133248) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : ARHGDIA knockout HEK293T cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 23 kDaab108977 was shown to react with aRHGDIA in wild-type HEK293T cells in Western blot with loss of signal observed in ARHGDIA knockout cell line ab266447 (ARHGDIA knockout cell lysate ab257356). Wild-type HEK293T and ARHGDIA knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab108977 overnight at 4 °C at a 1/1000 dilution. Blots were incubated with goat anti-rabbit HRP secondary antibodies at 0.2μg/mL before imaging.
These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.
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All lanes : Anti-RhoGDI antibody [EPR3772] (ab108977) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : ARHGDIA knockout HEK293T cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 23 kDa
Observed band size: 23 kDaLanes 1-4: Merged signal (red and green). Green - ab108977 observed at 23 kDa. Red - loading control ab8245 observed at 36 kDa.
ab108977 Anti-RhoGDI antibody [EPR3772] was shown to specifically react with RhoGDI in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266446 (knockout cell lysate ab257355) was used. Wild-type and RhoGDI knockout samples were subjected to SDS-PAGE. ab108977 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-RhoGDI antibody [EPR3772] (ab108977) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : ARHGDIA knockout HEK293T cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 23 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?Lanes 1-3: Merged signal (red and green). Green - ab108977 observed at 27 kDa. Red - loading control ab8245 observed at 36 kDa.
ab108977 Anti-RhoGDI antibody [EPR3772] was shown to specifically react with RhoGDI in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266447 (knockout cell lysate ab257356) was used. Wild-type and RhoGDI knockout samples were subjected to SDS-PAGE. ab108977 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-RhoGDI antibody [EPR3772] (ab108977) at 1/1000 dilution
Lane 1 : Jurkat cell lysates
Lane 2 : HeLa cell lysates
Lane 3 : HL-60 cell lysates
Lane 4 : NIH/3T3 cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 23 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab108977 has been referenced in 2 publications.
- Kempf SJ et al. The cognitive defects of neonatally irradiated mice are accompanied by changed synaptic plasticity, adult neurogenesis and neuroinflammation. Mol Neurodegener 9:57 (2014). WB . PubMed: 25515237
- Yentrapalli R et al. The PI3K/Akt/mTOR pathway is implicated in the premature senescence of primary human endothelial cells exposed to chronic radiation. PLoS One 8:e70024 (2013). Human . PubMed: 23936371