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RhoGDI protein (Tagged-His Tag) (ab91668)

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Overview

  • Product nameRhoGDI protein (Tagged-His Tag)See all RhoGDI proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 6-204 of Human RhoGDI with an N terminal His tag; MWt 25kDa (UniProt P52565).
  • Expression hostE. coli

    • Properties

  • Purification notes"Purity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation."
  • FormLyophilised:Reconstitute with 156 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Sequence notesPTAEQLAQIAAENEEDEHSVNYKPPAQKSIQEIQELDKDD ESLRKYKEAL LGRVAVSADPNVPNVVVTGLTLVCSSAP GPLELDLTGDLESFKKQSFVLK EGVEYRIKISFRVNRE IVSGMKYIQHTYRKGVKIDKTDYMVGSYGPRAEE YEFL TPVEEAPKGMLARGSYSIKSRFTDDDKTDHLSWEWNLTIK KDWKD
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab91668 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
  • Application notesSDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        ARHGDIAfa96g11GDIA 1
        GDIA1GDIR1_HUMANMGC117248Rho GDIRho GDI 1Rho GDI alphaRho GDP dissociation inhibitor (GDI) alphaRho GDP dissociation inhibitor 1Rho GDP dissociation inhibitor alphaRho GDP-dissociation inhibitor 1Rho-GDI alphaRHOGDIRhoGDI 1RhoGDI alphaRhoGDI1wu:fa96g11zgc:55554zgc:77681
      see all
  • FunctionRegulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them.
  • Sequence similaritiesBelongs to the Rho GDI family.
  • Cellular localizationCytoplasm.

    • Target information above from: UniProt accession P52565 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    RhoGDI protein (Tagged-His Tag) images

    • SDS-PAGE - RhoGDI protein (Tagged-His Tag) (ab91668)
      SDS-PAGE - RhoGDI protein (Tagged-His Tag) (ab91668)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for RhoGDI protein (Tagged-His Tag) (ab91668)

    ab91668 has not yet been referenced specifically in any publications.

    Publishing research using ab91668 ? Please let us know so that we can cite the reference in this datasheet

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    Sehr geehrte Damen und Herren, wir möchten in unserer Arbeitsgruppe beginnen, mit der NADPH-oxidase in Makrophagen (Rattenmakrophagen NR8383, ev. auch humane Monozyten/Makrophagen U937) zu arbeiten und sind auf der Suche nach Antikörpern gegen ihre Bestan...

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    Vielen Dank für Ihre Anfrage. Gegen die genannten NADPH-Oxidase-Komponenten haben wir folgende Antikörper bzw. Proteine in unserem Katalog: Click here (or use the following: http://www.abcam.com/index.html?datasheet=109366). Click here (or use the fo...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"