Rhod-4 Medium Removal No Wash Calcium Assay kit (ab112156)

Overview

  • Product nameRhod-4 Medium Removal No Wash Calcium Assay kitSee all Calcium kits ...
  • Sample type
    Adherent cells, Suspension cells
  • Assay typeQuantitative
  • Assay time
    1h 00m
  • Species reactivity
    Reacts with: Human
  • Product overview

    ab112156 provides an optimized assay method for monitoring the G-protein-coupled receptors and calcium channels. It can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation. Compared to Fluo-8, Rhod-4 is more photostable, making its fluorescence imaging more robust.

    Visit our FAQs page for tips and troubleshooting.

  • Notes

    This product is intended to use for monitoring calcium fluctuations in vivo in live cells. If you would like to quantify calcium concentration in vitro using cell extracts, we recommend using Calcium Detection Kit (Colorimetric) (ab102505) or Calcium Quantification Assay Kit (ab112115), as they provide stock standards for comparison.

  • Tested applicationsFunctional Studies more details

Properties

  • RelevanceCalcium is essential for all living organisms, where Ca2+ sequestration and release into and out of the cytoplasm functions as a signal for many cellular processes. 99% of calcium is found in bones and teeth with the remaining 1% found in the blood and soft tissue. Serum calcium levels are tightly controlled (8.4-11.4 mg/dL) and any variation outside this range can have serious effects. Calcium plays a role in mediating the constriction and relaxation of blood vessels, nerve impulse transmission, muscle contraction, and hormone secretion. Calcium ion channels control the migration of calcium ions across cell membranes, permitting the activation and inhibition of a wide variety of enzymes. Causes of low calcium levels include chronic kidney failure, vitamin D deficiency, and low blood magnesium levels that can occur in severe alcoholism.
  • Alternative names
    • Ca++
    • Ca2+

Applications

Our Abpromise guarantee covers the use of ab112156 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Functional Studies Use at an assay dependent concentration.

Rhod-4 Medium Removal No Wash Calcium Assay kit images

  • Carbachol Dose Response measured in HEK293 cells using Rhod-4 Medium Removal No Wash Calcium Assay kit and Rhod-2.

    HEK293 cells were incubated with 100 μL of the dye-loading solution using ab112156, or 100 μL of Rhod-2 solution (5 μM) for 1 hour at room temperature. Carbachol (25 μL/well) was added to achieve the final indicated concentrations. The EC50 of Rhod-4 is about 0.8 μM, and that for Rhod-2 about 0.7 μM.

Protocols

References for Rhod-4 Medium Removal No Wash Calcium Assay kit (ab112156)

ab112156 has not yet been referenced specifically in any publications.

Product Wall

The product is designed for plate reader based experiments, unfortunately we do not have direct experiences using flow cytometry with the kit. The kit should work though using Ex/Em = 540/590nm. Reviewing the internet, it seems that it is possible to u...

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Thank you for your inquiry.

1. if the kits for measuring intracellular calcium mobilization can work with mouse cell lines, specifically MLE-12 cell line?--Yes, as long as the cells contains calcium channels and Gq receptors

2. Also, ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"