Rhod-4 No Wash Calcium Assay kit (ab112157)
- Product nameRhod-4 No Wash Calcium Assay kitSee all Calcium kits ...
- Sample typeAdherent cells, Suspension cells
- Assay typeQuantitative
- Assay time1h 00m
- Species reactivityReacts with: Human
- Product overview
Abcam’s Calcium Assay Kits provide a homogeneous fluorescence-based assay for detecting the intracellular calcium mobilization. Cells expressing a GPCR of interest that signals through calcium are pre-loaded with our proprietary Rhod-4 which can cross cell membranes. Rhod-4 is the brightest red calcium indicator available for HTS screening. Once inside the cell, the lipophilic blocking groups of Rhod-4 are cleaved by non-specific cell esterases, resulting in a negatively charged fluorescent dye that stays inside the cells. Its fluorescence is greatly enhanced upon binding to calcium. When cells are stimulated with screening compounds, the receptor signals the release of intracellular calcium, which greatly increase the fluorescence of Rhod-4.
ab112157 provides an optimized assay method for monitoring G-protein-coupled receptors and calcium channels. The kit comes with all the essential components and an optimized protocol. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation.
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- Tested applicationsFunctional Studies more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 kit 10X Pluronic® F127 Plus 1 x 1ml HHBS 1 x 9ml Rhod-4 1 vial
Our Abpromise guarantee covers the use of ab112157 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Rhod-4 No Wash Calcium Assay kit images
Carbachol Dose Response was measured in HEK293 cells with ab112157 and Rhod-2. HEK293 cells were seeded overnight at 40,000 cells/100 µL/well in a black wall/clear bottom 96-well plate. The cells were incubated with 100 µL of dye-loading solution using ab112157, or 100 µL of Rhod-2 solution (5 µM) for 1 hour at room temperature. Carbachol (25 µL/well) was added to achieve the final indicated concentrations. The EC50 of Rhod-4 is about 0.6 µM, and that for Rhod-2 is about 0.7 µM.
References for Rhod-4 No Wash Calcium Assay kit (ab112157)
ab112157 has not yet been referenced specifically in any publications.