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Anti-Rhodopsin antibody [RET-P1] (ab3267)

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Overview

Product name

Anti-Rhodopsin antibody [RET-P1]
See all Rhodopsin products (15) ...

Description

Mouse monoclonal [RET-P1] to Rhodopsin

Specificity

This antibody specifically labels the axons andsynaptic pedicles of the rods. The epitope has not been determined.

Tested applications

ICC/IF, IHC-FrFl, IHC-P, WB, IP, IHC - Wholemountmore details

Cross reactivity

Reacts with

Mouse, Rat, Cow, Human

Immunogen

Membrane preparation from adult rat retina.

Positive control

IMR-5 cells Brain or retina tissue sections.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

10mM PBS, pH7.4, 0.2%BSA, 0.09% sodium azide

Concentration

Concentration information loading...

Purity

Protein G purified

Primary antibody notes

Rhodopsin is a glycoprotein which is a visual pigment in mammals, birds, amphibians, and reptiles. The rhodopsin protein is present on the photoreceptor cells in the retina.

Clonality

Monoclonal

Clone number

RET-P1

Isotype

IgG1

Research areas

Neuroscience >> Sensory System >> Visual system
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> GPCR

  • - Anti-Rhodopsin antibody [RET-P1] (ab3267)- Anti-Rhodopsin antibody [RET-P1] (ab3267) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab3267 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

IP: Use at an assay dependent dilution (PMID 18974094).
ICC/IF: Use at an assay dependent dilution.
IHC-P: 1/2 - 1/4. Perform heat mediated antigen retrieval before commencing with IHC: staining protocol (boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 min).
WB: Use at a concentration of 1 - 2 µg/ml for 2hrs at RT. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change leading to G-protein activation and release of all-trans retinal.

Tissue specificity

Rod shaped photoreceptor cells which mediates vision in dim light.

Involvement in disease

Defects in RHO are the cause of retinitis pigmentosa type 4 (RP4) [MIM:613731]. RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well.
Defects in RHO are the cause of congenital stationary night blindness autosomal dominant type 1 (CSNBAD1) [MIM:610445]; also known as rhodopsin-related congenital stationary night blindness. Congenital stationary night blindness is a non-progressive retinal disorder characterized by impaired night vision.

Sequence similarities

Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.

Post-translational
modifications

Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region.
Contains one covalently linked retinal chromophore.

Cellular localization

Membrane. Synthesized in the inner segment (IS) of rod photoreceptor cells before vectorial transport to the rod outer segment (OS) photosensory cilia.

Target information above from: UniProt accessionP08100 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • CSNBAD1 antibody
  • MGC138309 antibody
  • MGC138311 antibody
  • OPN 2 antibody
  • OPN2 antibody
  • opsd antibody
  • OPSD_HUMAN antibody
  • opsin 2 antibody
  • Opsin 2 antibody
  • Opsin 2 rod pigment antibody
  • Opsin-2 antibody
  • Opsin2 antibody
  • Retinitis Pigmentosa 4 antibody
  • Retinitis pigmentosa 4 autosomal dominant antibody
  • RHO antibody
  • Rhodopsin antibody
  • RP 4 antibody
  • RP4 antibody
see all

Database links

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Anti-Rhodopsin antibody [RET-P1] images:

  - Anti-Rhodopsin antibody [RET-P1] (ab3267)

- Anti-Rhodopsin antibody [RET-P1] (ab3267)

ab3267 at a 1/100 dilution staining Rhosopsin in murine retina by IHC-Wholemount. Tissue was fixed in 4% paraformaldehyde and permeabilized using Triton. The secondary used was an Atto488 conjugated goat polyclonal used at a 1/200 dilution.

Rods stained with ab3267 (green) in whole mount retina of young adult mouse (p21), actin stained using phalloidin (red).

Image courtesy of Dr Vincent Michel by Abreview.

See Abreview

References for Anti-Rhodopsin antibody [RET-P1] (ab3267)

This product has been referenced in:

  • Vekslin S & Ben-Yosef T Spatiotemporal expression pattern of ceramide kinase-like in the mouse retina. Mol Vis 16:2539-49 (2010). IHC-P; Mouse.Read more (PubMed: 21151604) »
  • Meira LB  et al. Aag-initiated base excision repair drives alkylation-induced retinal degeneration in mice. Proc Natl Acad Sci U S A 106:888-93 (2009). IHC-P; Mouse.Read more (PubMed: 19139400) »

See all 5 publications for this product

Publishing research using ab3267? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"