Anti-Ribonuclease H2, subunit A/RNASEH2A antibody (ab228788)
Key features and details
- Rabbit polyclonal to Ribonuclease H2, subunit A/RNASEH2A
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Ribonuclease H2, subunit A/RNASEH2A antibody
See all Ribonuclease H2, subunit A/RNASEH2A primary antibodies -
Description
Rabbit polyclonal to Ribonuclease H2, subunit A/RNASEH2A -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cow, Pig -
Immunogen
Recombinant fragment within Human Ribonuclease H2, subunit A/RNASEH2A (internal sequence). The exact sequence is proprietary.
Database link: O75792 -
Positive control
- WB: HeLa whole cell and nuclear extracts. ICC/IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab228788 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 33 kDa.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 33 kDa. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Catalytic subunit of RNase HII, an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. Participates in DNA replication, possibly by mediating the removal of lagging-strand Okazaki fragment RNA primers during DNA replication. Mediates the excision of single ribonucleotides from DNA:RNA duplexes. -
Involvement in disease
Aicardi-Goutieres syndrome 4 -
Sequence similarities
Belongs to the RNase HII family. Eukaryotic subfamily. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 10535 Human
- SwissProt: O75792 Human
- Unigene: 532851 Human
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Alternative names
- AGS4 antibody
- Aicardi Goutieres syndrome 4 protein antibody
- Aicardi-Goutieres syndrome 4 protein antibody
see all
Images
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4% paraformaldehyde fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Ribonuclease H2, subunit A/RNASEH2A (green) using ab228788 at 1/200 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue). Phalloidin, a cytoskeleton marker, was labeled 1/200 dilution.
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All lanes : Anti-Ribonuclease H2, subunit A/RNASEH2A antibody (ab228788) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) nuclear extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 33 kDa12% SDS-PAGE gel.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab228788 has not yet been referenced specifically in any publications.