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Western blot - Ribophorin I antibody (ab38451)
(enlarge)
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Product Name
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Ribophorin I antibody
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See all Ribophorin I antibodies (6)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Ribophorin I
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Immunogen
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Synthetic peptide conjugated to KLH (10-30 aa in length) at the C-term of last 50 aa of human ribophorin 1.
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Reacts with
(species key)
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Hu, Ms
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/1000.
WB: 1/100 - 1/500. Predicted molecular weight: 69 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HeLa cell lysate.
Mouse liver tissue lysate.
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Cellular localization
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Type I membrane protein in the endoplasmic reticulum.
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Research areas
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Chromatin and Nuclear Signaling >> DNA / RNA >> Translation >> Ribosome
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Relevance
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Ribophorins I and II represent proteins that appear to be involved in ribosome binding. They are abundant, highly conserved glycoproteins located exclusively in the membranes of the rough endoplasmic reticulum. Ribophorin I forms a complex with ribophorin II, OST48 and DAD1 in the endoplasmic reticulum, which has oligosaccharyltransferase activity.
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Ribophorin I antibody (ab38451) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein G purified
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Storage buffer
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Preservative: 0.09% Sodium Azide
Constituents: PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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This antibody is purified through a protein G column, eluted out with both high and low pH buffers, neutralized immediately after elution then followed by dialysis against PBS.
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Form
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Liquid
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Concentration
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0.250 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Ribophorin I antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Ribophorin I antibody images, references, products related to ab38451 and other tools.
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Ribophorin I antibody images:
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 Western blot - Ribophorin I antibody (ab38451)
All lanes : Ribophorin I antibody (ab38451) at 1/100 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Mouse liver tissue lysate
Predicted band size : 69 kDa
Observed band size : 69 kDa
Additional bands at : 32 kDa,50 kDa. We are unsure as to the identity of these extra bands.
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Search PubMed (MEDLINE) for references to Ribophorin I
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Ribophorin I antibody - more information
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Customer reviews (feedback) regarding Ribophorin I antibody |
Customer FAQs regarding Ribophorin I antibody |
Protocols for Ribophorin I antibody |
Price and availability of products related to Ribophorin I antibody |
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Ribophorin I antibody - related products:
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Ribophorin I antibody - other tools:
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