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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Ribosome
Overview
Product name
Anti-Ribophorin I antibody
See all Ribophorin I products (7) ...
Description
Rabbit polyclonal to Ribophorin I
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide conjugated to KLH (10-30 aa in length) at the C-term of last 50 aa of human ribophorin 1.
Positive control
HeLa cell lysate. Mouse liver tissue lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
This antibody is purified through a protein G column, eluted out with both high and low pH buffers, neutralized immediately after elution then followed by dialysis against PBS.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Ribosome
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Western blot - Ribophorin I antibody (ab38451)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab38451 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/1000ELISA: 1/1000
WB: 1/100 - 1/500.Predicted molecular weight: 69 kDa.
Target
Function
Essential subunit of N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains.
Tissue specificity
Expressed in all tissues tested.
Sequence similarities
Belongs to the OST1 family.
Cellular localization
Endoplasmic reticulum membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Target information above from: UniProt accessionP04843
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- D6Wsu137e antibody
- Dolichyl diphosphooligosaccharide protein glycosyltransferase 67 kDa subunit precursor antibody
- Dolichyl-diphosphooligosaccharide protein glycosyltransferase 67 kDa subunit precursor antibody
see all
Database links
- Entrez Gene: 6184 Human
- Entrez Gene: 103963 Mouse
- Omim: 180470 Human
- SwissProt: P04843 Human
- SwissProt: Q6IBR0 Human
- SwissProt: Q91YQ5 Mouse
- Unigene: 518244 Human
- Unigene: 603636 Human
see all
Anti-Ribophorin I antibody images:
Western blot - Ribophorin I antibody (ab38451)
All lanes : Anti-Ribophorin I antibody (ab38451) at 1/100 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Mouse liver tissue lysate
Predicted band size : 69 kDa
Observed band size : 69 kDa
Additional bands at : 32 kDa,50 kDa. We are unsure as to the identity of these extra bands.
References for Anti-Ribophorin I antibody (ab38451)
ab38451 has not yet been referenced specifically in any publications.
Publishing research using ab38451? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"