Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Ribosome
Overview
Product name
Anti-Ribophorin I antibody
See all Ribophorin I products (7) ...
Description
Rabbit polyclonal to Ribophorin I
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Chicken, Cow, Dog, Pig, Zebrafish
Immunogen
Synthetic peptide within residues: AAEARMKVAC ITEQVLTLVN KRIGLYRHFD ETVNRYKQSR DISTLNSGKK , corresponding to internal sequence amino acids 467-516 of Human Ribophorin I (NP_002941)
Positive control
Transfected 293T cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab86267 is purified by a peptide affinity chromatography method.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Ribosome
Applications
Show applications key
Our Abpromise guarantee covers the use of ab86267 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 66 kDa (predicted molecular weight: 66 kDa).
ICC/IF: Use a concentration of 5 µg/ml
Target
Function
Essential subunit of N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains.
Tissue specificity
Expressed in all tissues tested.
Sequence similarities
Belongs to the OST1 family.
Cellular localization
Endoplasmic reticulum membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Target information above from: UniProt accessionP04843
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- D6Wsu137e antibody
- Dolichyl diphosphooligosaccharide protein glycosyltransferase 67 kDa subunit precursor antibody
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit antibody
see all
Database links
- Entrez Gene: 6184 Human
- Entrez Gene: 103963 Mouse
- Entrez Gene: 397606 Pig
- Entrez Gene: 25596 Rat
- Entrez Gene: 325561 Zebrafish
- Omim: 180470 Human
- SwissProt: P04843 Human
- SwissProt: Q6IBR0 Human
see all
Anti-Ribophorin I antibody images:
Western blot - Ribophorin I antibody (ab86267)
Anti-Ribophorin I antibody (ab86267) at 1 µg/ml + Transfected 293T cell lysate at 10 µg
Secondary
anti-Rabbit IgG HRP at 1/50000 dilution
Predicted band size : 66 kDa
Observed band size : 66 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Ribophorin I antibody (ab86267)
ICC/IF image of ab86267 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab86267, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-Ribophorin I antibody (ab86267)
ab86267 has not yet been referenced specifically in any publications.
Publishing research using ab86267? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"