Overview

  • Product name
    Ro 31-8220 mesylate (Bisindolylmaleimide IX)
  • Description
    Protein kinase inhibitor
  • Alternative names
    • Bisindolylmaleimide IX
  • Biological description
    Widely used as a cell-permeable, reversible, competitive protein kinase C inhibitor. Also potently inhibits wide range of kinases inlcuding RSK1, RSK2, PRK2, PKCα, PKD1, MSK1, GSK3β, CDK2-cyclin A and PIM3, as well as PKCα.

    Also available in simple stock solutions (ab146723) - add 1 ml of water to get an exact, ready-to-use concentration.
  • Purity
    > 98%

Properties

Images

  • 1.5 x 10e7 THP-1 cells were incubated with 100 nM GF 109203X (ab144264) or Ro 31-8220 mesylate (ab120374) for 30 minutes prior to activation with 10 µg x mL-1 PMA (Sigma) for 4 hours. Control cells were left without inhibitors or PMA. Cells were lysed in 1 mL of lysis buffer, and 30 µL were tested for PKC activity (duplicates; +/- SD).

References

This product has been referenced in:
  • Bain J  et al. The selectivity of protein kinase inhibitors: a further update. Biochem J 408:297-315 (2007). Read more (PubMed: 17850214) »
  • Davis PD  et al. Inhibitors of protein kinase C. 2. Substituted bisindolylmaleimides with improved potency and selectivity. J Med Chem 35:994-1001 (1992). Read more (PubMed: 1552513) »
  • Dieter P & Fitzke E RO 31-8220 and RO 31-7549 show improved selectivity for protein kinase C over staurosporine in macrophages. Biochem Biophys Res Commun 181:396-401 (1991). Read more (PubMed: 1958208) »

See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab120374.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"

Sign up