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Product name
Anti-PSMD1 antibody
See all PSMD1 products (7) ...
Description
Chicken polyclonal to PSMD1
Tested applications
Cross reactivity
Reacts with
Rat, Cow, Dog, Human, Chimpanzee
Immunogen
Synthetic peptide, corresponding to N terminal amino acids 21-270 of Human Rpn2.
Positive control
E coli derived fusion protein.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgY
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Proteasome
-
Western blot - Rpn2 antibody (ab37759)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab37759 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: 1/2000. Detects a band of approximately 80 kDa for the fusion protein (predicted molecular weight for the native protein: 106 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Acts as a regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.
Sequence similarities
Belongs to the proteasome subunit S1 family.
Contains 10 PC repeats.
Target information above from: UniProt accessionQ99460
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- MGC133041 antibody
- 26S proteasome non ATPase regulatory subunit 1 antibody
- 26S proteasome non ATPase subunit 1 antibody
see all
Database links
- Entrez Gene: 5707 Human
- Entrez Gene: 83806 Rat
- SwissProt: Q99460 Human
- SwissProt: O88761 Rat
- Unigene: 3887 Human
- Unigene: 2891 Rat
Anti-PSMD1 antibody images:
Western blot - Rpn2 antibody (ab37759)
Anti-PSMD1 antibody (ab37759) at 1/2000 dilution + E coli derived fusion protein at 2.5 µg
Secondary
Goat anti IgY HRP at 1/1000 dilution
Predicted band size : 106 kDa
Observed band size : 80 kDa (why is the actual band size different from the predicted?)
The arrow next to the image points to the Rpn2 fusion protein.
Observed band size may differ from predicted band size due to the test antigen being a fusion protein.
Colorimetric method was used for signal development.
References for Anti-PSMD1 antibody (ab37759)
ab37759 has not yet been referenced specifically in any publications.
Publishing research using ab37759? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"