Anti-Rubella Virus capsid antibody (ab34749)
- Product nameAnti-Rubella Virus capsid antibody
- DescriptionMouse monoclonal to Rubella Virus capsid
- SpecificityThis antibody does not react with measles and mumps virus nucleocapsid proteins.
- Tested applicationsIndirect ELISA, WB more details
- Species reactivityReacts with rubella. Not yet tested in other species.
Recombinant full length protein - purified rubella virus capsid protein expressed in yeast -S.cerevisiae. To produce the recombinant antigen, RNA was isolated from Rubella virus strain RA27/3 (GenBank: X14871.1).
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.4
- Concentration information loading...
- PurityIgG fraction
- Clonality Monoclonal
- Research Areas
Our Abpromise guarantee covers the use of ab34749 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Indirect ELISA||I-ELISA: 1/1000 - 1/10000.|
|WB||WB: 1/1000 - 1/5000. Predicted molecular weight: 115 kDa.|
- RelevanceRubella virus is the only member of the Rubrivirus genus of the Togavirus family. Unlike most Togaviruses it is NOT arthropod borne, but is acquired via the respiratory route. It causes German measles ( a mild contagious eruptive disease, capable of producing congenital defects in infants born to mothers infected during the first three months of pregnancy). Rubella virus is an enveloped positive-strand RNA virus. The genome encodes two open reading frames (ORFs): the 5'-proximal ORF encodes viral nonstructural proteins (NSP) that are responsible for viral genome replication, while the 3'-proximal ORF encodes three virion structural proteins (SP), the capsid protein (CP), and the two envelope glycoproteins, E2 and E1. During virus assembly, the capsid interacts with genomic RNA to form nucleocapsids. The rubella virus (RV) structural proteins: capsid, E2, and E1 are synthesized as a polyprotein precursor. The signal peptide that initiates translocation of E2 into the lumen of the endoplasmic reticulum remains attached to the carboxy terminus of the capsid protein after cleavage by signal peptidase.
- Cellular localizationCytoplasmic in host cells concentrated around Golgi region and mitochondrion.
- Coat protein antibody
- p110 antibody
- rubella capsid antibody
- Structural polyprotein antibody
Anti-Rubella Virus capsid antibody images
All lanes : Anti-Rubella Virus capsid antibody (ab34749) at 1 µg/ml
Lane 1 : Prestained M.Wt markers
Lane 2 : crude yeast cell lysate [mock control]
Lane 3 : irrelevant viral proteins
Lane 4 : irrelevant viral proteins
Lane 5 : recombinant purified rubella capsid protein
Lane 6 : crude lysate of transformed yeast cells expressing rubella capsid protein
Predicted band size : 115 kDa
References for Anti-Rubella Virus capsid antibody (ab34749)
ab34749 has not yet been referenced specifically in any publications.