Anti-Rubella Virus capsid antibody (ab34749)


  • Product nameAnti-Rubella Virus capsid antibody
  • Description
    Mouse monoclonal to Rubella Virus capsid
  • SpecificityThis antibody does not react with measles and mumps virus nucleocapsid proteins.
  • Tested applicationsSuitable for: Indirect ELISA, WBmore details
  • Species reactivity
    Reacts with rubella. Not yet tested in other species.
  • Immunogen

    Recombinant full length protein - purified rubella virus capsid protein expressed in yeast -S.cerevisiae. To produce the recombinant antigen, RNA was isolated from Rubella virus strain RA27/3 (GenBank: X14871.1).


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • PurityIgG fraction
  • ClonalityMonoclonal
  • MyelomaSp2/0
  • IsotypeIgG1
  • Research areas


Our Abpromise guarantee covers the use of ab34749 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Indirect ELISA 1/1000 - 1/10000.
WB 1/1000 - 1/5000. Predicted molecular weight: 115 kDa.


  • RelevanceRubella virus is the only member of the Rubrivirus genus of the Togavirus family. Unlike most Togaviruses it is NOT arthropod borne, but is acquired via the respiratory route. It causes German measles ( a mild contagious eruptive disease, capable of producing congenital defects in infants born to mothers infected during the first three months of pregnancy). Rubella virus is an enveloped positive-strand RNA virus. The genome encodes two open reading frames (ORFs): the 5'-proximal ORF encodes viral nonstructural proteins (NSP) that are responsible for viral genome replication, while the 3'-proximal ORF encodes three virion structural proteins (SP), the capsid protein (CP), and the two envelope glycoproteins, E2 and E1. During virus assembly, the capsid interacts with genomic RNA to form nucleocapsids. The rubella virus (RV) structural proteins: capsid, E2, and E1 are synthesized as a polyprotein precursor. The signal peptide that initiates translocation of E2 into the lumen of the endoplasmic reticulum remains attached to the carboxy terminus of the capsid protein after cleavage by signal peptidase.
  • Cellular localizationCytoplasmic in host cells concentrated around Golgi region and mitochondrion.
  • Alternative names
    • Coat protein antibody
    • p110 antibody
    • rubella capsid antibody
    • Structural polyprotein antibody

Anti-Rubella Virus capsid antibody images

  • All lanes : Anti-Rubella Virus capsid antibody (ab34749) at 1 µg/ml

    Lane 1 : Prestained M.Wt markers
    Lane 2 : crude yeast cell lysate [mock control]
    Lane 3 : irrelevant viral proteins
    Lane 4 : irrelevant viral proteins
    Lane 5 : recombinant purified rubella capsid protein
    Lane 6 : crude lysate of transformed yeast cells expressing rubella capsid protein

    Predicted band size : 115 kDa

References for Anti-Rubella Virus capsid antibody (ab34749)

This product has been referenced in:
  • Perelygina L  et al. Rubella persistence in epidermal keratinocytes and granuloma M2 macrophages in patients with primary immunodeficiencies. J Allergy Clin Immunol 138:1436-1439.e11 (2016). IF . Read more (PubMed: 27613149) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BlockAid as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 20°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric buffer, pH 6
Sample Human Tissue sections (Lung)
Specification Lung
Permeabilization Yes - 0.2% Triton X-100
Fixative Formaldehyde

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Verified customer

Submitted Apr 10 2015

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Sample Human Cell (HUVEC)
Specification HUVEC
Permeabilization No
Fixative Methanol

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Verified customer

Submitted Apr 10 2015

Application Western blot
Loading amount 10 µg
Gel Running Conditions Reduced Denaturing (4-12% PAGE)
Sample African Green Monkey Cell lysate - whole cell (Vero cells)
Specification Vero cells
Treatment infected with rubella virus
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Apr 10 2015

Thank you for contacting us.  I am still waiting for additional information regarding the strain used to generate ab106292, but I will let you know as soon as I hear back from the lab regarding this.  The other products are as follows: ab34749: 9B11 wa...

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