Overview
Product nameRubella Virus capsid proteinSee all Rubella Virus capsid proteins and peptides ...
Protein descriptionRecombinant full length Rubella Virus capsid protein expressed in S. cerevisiae, 35kDa.
Expression hostSaccharomyces cerevisiae
Properties
Purity> 90
% by SDS-PAGE
Purification notesPurified by ultracentifugation.
FormLyophilised:Reconstitute with PBS or 8M urea (to obtain 0.5 mg/ml protein stock solution). After reconstitution in PBS store at -20°C. Avoid freeze / thaw cycles. Proteins reconstituted in 8M urea store at 4°C.
Storage instructionsStore at +4°C.
Storage bufferPreservative: None
Constituents: PBS
Concentration information loading...
Research Areas
Applications
Our Abpromise guarantee covers the use of
ab74574
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Application
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Notes |
| SDS-PAGE |
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| ELISA |
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| WB |
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Application notesELISA: Use at a concentration of 2 µg/ml to coat a microtitre plate..
WB: Use at a concentration of 1 - 2 µg/lane. Predicted molecular weight: 33 kDa.
SDS-PAGE: Use at a concentration of 2 µg/lane.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Protein info
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Alternative names
Coat proteingerman measlesgerman measles capsid protein
p110rubella capsidStructural polyprotein
see all
RelevanceRubella virus is the only member of the Rubrivirus genus of the Togavirus family. Unlike most Togaviruses it is NOT arthropod borne, but is acquired via the respiratory route. It causes German measles ( a mild contagious eruptive disease, capable of producing congenital defects in infants born to mothers infected during the first three months of pregnancy).
Rubella virus is an enveloped positive-strand RNA virus. The genome encodes two open reading frames (ORFs): the 5'-proximal ORF encodes viral nonstructural proteins (NSP) that are responsible for viral genome replication, while the 3'-proximal ORF encodes three virion structural proteins (SP), the capsid protein (CP), and the two envelope glycoproteins, E2 and E1. During virus assembly, the capsid interacts with genomic RNA to form nucleocapsids. The rubella virus (RV) structural proteins: capsid, E2, and E1 are synthesized as a polyprotein precursor. The signal peptide that initiates translocation of E2 into the lumen of the endoplasmic reticulum remains attached to the carboxy terminus of the capsid protein after cleavage by signal peptidase.
Cellular localizationCytoplasmic in host cells concentrated around Golgi region and mitochondrion.
Rubella Virus capsid protein images
References for Rubella Virus capsid protein (ab74574)
ab74574
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
SDS-PAGE - Rubella Virus capsid protein (ab74574)SDS-PAGE showing ab74574 at approximately 35kDa (2µg/lane)