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Anti-Ryanodine Receptor antibody [34C] (ab2868)

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Overview

Product name

Anti-Ryanodine Receptor antibody [34C]
See all Ryanodine Receptor products (7) ...

Description

Mouse monoclonal [34C] to Ryanodine Receptor

Specificity

Detects Ryanodine Receptor (RyR)-1 and RyR-2 isoforms. In chickens, this antibody detects the alpha, beta and cardiac isoforms. This antibody detects RyR-3 in mouse cells. In frog, this antibody detects the alpha and beta isoforms. In fish, this antibody detects the alpha isoform. By Western blot, this antibody detects a 565 kDa protein representing RyR from rat skeletal muscle extracts. In non-mammalian vertebrates, a doublet is seen at 565 kDa representing the alpha and beta isoforms of the receptor. Immunohistochemical staining of RyR in chicken brain results in intense staining of cerebellar Purkinje neurons.

Tested applications

ICC/IF, IHC-P, IHC-Fr, IP, WBmore details

Cross reactivity

Reacts with

Mouse, Rat, Sheep, Rabbit, Cow, Dog, Human

Predicted to work with

Fish, Amphibians

Immunogen

Partially purified chicken pectoral muscle ryanodine receptor.

Positive control

rat skeletal muscle

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

PBS with 0.05% sodium azide

Concentration

Concentration information loading...

Purity

Ascites

Primary antibody notes

The Ryanodine Receptor (RyR) is the channel responsible for calcium release from muscle cell Sarcoplasmic Reticulum (SR) and also plays a role in calcium regulation in non-muscle cells. The RyR exists as a homotetramer and is predicted to have a short cytoplasmic C-terminus and 4-10 transmembrane domains. The remainder of the protein, termed the "foot" region, is located in the cytoplasm between the transverse tubule and the SR. Mammalian RyR isoforms are the product of three different genes: RyR-1 is expressed predominantly in skeletal muscle and areas of the brain; RyR-2 is expressed predominantly in heart muscle but also found in the stomach, endothelial cells and diffuse areas of the brain; and RyR-3 is found in smooth muscle and the brain (striatum, thalamus and hippocampus). In non-mammalian vertebrates, the RyR isoforms are termed alpha, beta and cardiac which correlate loosely to the mammalian RyR-1, RyR-3 and RyR-2 isoforms respectively.

Clonality

Monoclonal

Clone number

34C

Isotype

IgG1

Research areas

Cancer >> Cancer Metabolism >> Response to hypoxia
Cardiovascular >> Heart >> Contractility >> Inotropics
Cardiovascular >> Heart >> Cardiac arrhythmias
Neuroscience >> Neurotransmission >> Calcium Signaling >> Calcium Channels >> Ryanodine Receptors

  • Immunocytochemistry/ Immunofluorescence - Ryanodine Receptor antibody [34C] (ab2868)Immunocytochemistry/ Immunofluorescence - Ryanodine Receptor antibody [34C] (ab2868) image (enlarge)

  • Immunohistochemistry (Frozen sections) - Ryanodine Receptor antibody [34C] (ab2868)Immunohistochemistry (Frozen sections) - Ryanodine Receptor antibody [34C] (ab2868) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab2868 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

The Ryanodine Receptor (RyR) is the channel responsible for calcium release from muscle cell Sarcoplasmic Reticulum (SR) and also plays a role in calcium regulation in non-muscle cells. The RyR exists as a homotetramer and is predicted to have a short cytoplasmic C-terminus and 4-10 transmembrane domains. The remainder of the protein, termed the "foot" region, is located in the cytoplasm between the transverse tubule and the SR. Mammalian RyR isoforms are the product of three different genes: RyR-1 is expressed predominantly in skeletal muscle and areas of the brain; RyR-2 is expressed predominantly in heart muscle but also found in the stomach, endothelial cells and diffuse areas of the brain; and RyR-3 is found in smooth muscle and the brain (striatum, thalamus and hippocampus). In non-mammalian vertebrates, the RyR isoforms are termed alpha, beta and cardiac which correlate loosely to the mammalian RyR-1, RyR-3 and RyR-2 isoforms respectively. Defects in the RYR2 gene are the cause of several heart diseases: 1) familial arrhythmogenic right ventricular dysplasia 2 (ARVD2), also known as arrhythmogenic right ventricular cardiomyopathy 2 (ARVC2), 2) an autosomal dominant form of stress-induced polymorphic ventricular tachycardia (VTSIP), also known as catecholaminergic polymorphic ventricular tachycardia (CPVT) and 3) familial polymorphic ventricular tachycardia (FPVT). Ryanodine Receptor 2 contains several phosphorylatable sites. Specifically, Ser-2030 and Ser-2809 (or at Ser-2808 depending on the species) can be phosphoryated by protein kinase A (PKA) and Ser-2815 (or at Ser-2814 depending on the species) can be phosphorylated by CaMKII (Ca2+/calmodulin-dependent protein kinase II).

Cellular localization

Membrane; Multi-pass membrane protein

Alternative names

  • Arrhythmogenic right ventricular dysplasia 2 antibody
  • ARVC 2 antibody
  • ARVC2 antibody
  • ARVD 2 antibody
  • ARVD2 antibody
  • Brain ryanodine receptor calcium release channel antibody
  • Brain type ryanodine receptor antibody
  • Cardiac muscle ryanodine receptor antibody
  • Cardiac muscle ryanodine receptor calcium release channel antibody
  • Cardiac muscle type ryanodine receptor antibody
  • CCO antibody
  • Central core disease of muscle antibody
  • HBRR antibody
  • hRYR 2 antibody
  • hRYR2 antibody
  • MHS antibody
  • MHS1 antibody
  • Ryanodine receptor 1 (skeletal) antibody
  • Ryanodine receptor 1 antibody
  • Ryanodine receptor 2 (cardiac) antibody
  • Ryanodine receptor 2 antibody
  • Ryanodine receptor 3 antibody
  • Ryanodine receptor type1 antibody
  • RYDR antibody
  • RYR 1 antibody
  • RYR 2 antibody
  • RYR 3 antibody
  • RYR antibody
  • RYR1 antibody
  • RYR2 antibody
  • RYR3 antibody
  • Sarcoplasmic reticulum calcium release channel antibody
  • Skeletal muscle calcium release channel antibody
  • Skeletal muscle ryanodine receptor antibody
  • Skeletal muscle type ryanodine receptor antibody
  • SKRR antibody
  • Type 1 like ryanodine receptor antibody
  • VTSIP antibody
see all

Database links

see all

Anti-Ryanodine Receptor antibody [34C] images:

  Immunocytochemistry/ Immunofluorescence - Ryanodine Receptor antibody [34C] (ab2868)

Immunocytochemistry/ Immunofluorescence - Ryanodine Receptor antibody [34C] (ab2868)

ICC/IF image of ab2868 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2868, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  Immunohistochemistry (Frozen sections) - Ryanodine Receptor antibody [34C] (ab2868)

Immunohistochemistry (Frozen sections) - Ryanodine Receptor antibody [34C] (ab2868)

ab2868 at a 1/200 dilution staining Ryanodine Receptor in mouse liver tissue sections by Immunohistochemistry (frozen sections) incubated for 9 hours at +4°C. Fixed in formaldehyde, permeabilized using 0.2% Triton X-100. Blocked using 2% BSA for 30 minutes at 20°C. Secondary used at a 1/200 dilution polyclonal Goat anti-mouse IgG conjugated to Alexa Fluor 555.

This image is courtesy of an anonymous abreview.

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References for Anti-Ryanodine Receptor antibody [34C] (ab2868)

This product has been referenced in:

  • Lostal Wet al. Efficient recovery of dysferlin deficiency by dual adeno-associated vector-mediated gene transfer. Hum Mol Genet 19:1897-907 (2010). IHC-Fr; Mouse.Read more (PubMed: 20154340) »
  • Pfeffer Met al. The mammalian molecular clockwork controls rhythmic expression of its own input pathway components. J Neurosci 29:6114-23 (2009).Read more (PubMed: 19439589) »

See all 16 publications for this product

Publishing research using ab2868? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"