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Anti-Ryanodine Receptor antibody [C3-33] (ab2827)

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Overview

Product name

Anti-Ryanodine Receptor antibody [C3-33]
See all Ryanodine Receptor products (7) ...

Description

Mouse monoclonal [C3-33] to Ryanodine Receptor

Specificity

Detects ryanodine receptor 2 (RyR 2) and weakly detects RyR 1.

Tested applications

ICC/IF, IHC-Fr, Flow Cyt, IHC-P, IP, WBmore details

Cross reactivity

Reacts with

Rat, Rabbit, Chicken, Guinea pig, Dog, Human

Predicted to work with

Fish, Amphibians

Immunogen

Canine cardiac ryanodine receptor

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

PBS with 0.05% sodium azide

Concentration

Concentration information loading...

Purity

Ascites

Primary antibody notes

The Ryanodine Receptor (RyR) is the channel responsible for the release of calcium from the sarcoplasmic reticulum (SR) in muscle cells and also plays a role in calcium regulation in non-muscle cells. The RyR exists as a homotetramer and is predicted to have a short cytoplasmic C-terminus and 4-10 transmembrane domains; the remainder of the protein, termed the "foot" region is located in the cytoplasm between the T-tubule and the SR. The mammalian RyR is the product of three different genes: RyR 1, which is expressed predominantly in skeletal muscle and areas of the brain, RyR 2, which is expressed predominantly in the heart muscle but also found in the stomach, endothelial cells and diffuse areas of the brain, and RyR-3 which is found in smooth muscle and the brain (striatum, thalamus and hippocampus).

Clonality

Monoclonal

Clone number

C3-33

Isotype

IgG1

  • Immunohistochemistry (Frozen sections) - Ryanodine Receptor antibody [C3-33] (ab2827)Immunohistochemistry (Frozen sections) - Ryanodine Receptor antibody [C3-33] (ab2827) image (enlarge)

  • Immunohistochemistry (Frozen sections) - Ryanodine Receptor antibody [C3-33] (ab2827)Immunohistochemistry (Frozen sections) - Ryanodine Receptor antibody [C3-33] (ab2827) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab2827 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ICC/IF: Use at an assay dependent concentration.

  • IHC-Fr: Use a concentration of 1 µg/ml

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    Flow Cyt

     Flow Cyt: Use at an assa...Read more →

    Flow Cyt: Use at an assay dependent dilution.

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    IHC-P

     IHC-P: Use a concentrati...Read more →

    IHC-P: Use a concentration of 1 µg/ml(Staining of RyR in rat cardiac tissue results in intense staining of the myofiber, which is consistent with sarcoplasmic reticulum localization.)

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    IP

     IP: Use at an assay depe...Read more →

    IP: Use at an assay dependent dilution.

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    WB

     WB: Use a concentration ...Read more →

    WB: Use a concentration of 1 µg/ml(This antibody detects a 565 kDa protein representing the ryanodine receptor. In non-mammalian vertebrates, a doublet is seen at 565 kDa representing the alpha and beta isoforms of the receptor.)

Target

Relevance

The Ryanodine Receptor (RyR) is the channel responsible for calcium release from muscle cell Sarcoplasmic Reticulum (SR) and also plays a role in calcium regulation in non-muscle cells. The RyR exists as a homotetramer and is predicted to have a short cytoplasmic C-terminus and 4-10 transmembrane domains. The remainder of the protein, termed the "foot" region, is located in the cytoplasm between the transverse tubule and the SR. Mammalian RyR isoforms are the product of three different genes: RyR-1 is expressed predominantly in skeletal muscle and areas of the brain; RyR-2 is expressed predominantly in heart muscle but also found in the stomach, endothelial cells and diffuse areas of the brain; and RyR-3 is found in smooth muscle and the brain (striatum, thalamus and hippocampus). In non-mammalian vertebrates, the RyR isoforms are termed alpha, beta and cardiac which correlate loosely to the mammalian RyR-1, RyR-3 and RyR-2 isoforms respectively. Defects in the RYR2 gene are the cause of several heart diseases: 1) familial arrhythmogenic right ventricular dysplasia 2 (ARVD2), also known as arrhythmogenic right ventricular cardiomyopathy 2 (ARVC2), 2) an autosomal dominant form of stress-induced polymorphic ventricular tachycardia (VTSIP), also known as catecholaminergic polymorphic ventricular tachycardia (CPVT) and 3) familial polymorphic ventricular tachycardia (FPVT). Ryanodine Receptor 2 contains several phosphorylatable sites. Specifically, Ser-2030 and Ser-2809 (or at Ser-2808 depending on the species) can be phosphoryated by protein kinase A (PKA) and Ser-2815 (or at Ser-2814 depending on the species) can be phosphorylated by CaMKII (Ca2+/calmodulin-dependent protein kinase II).

Cellular localization

Membrane; Multi-pass membrane protein

Alternative names

  • Arrhythmogenic right ventricular dysplasia 2 antibody
  • ARVC 2 antibody
  • ARVC2 antibody
  • ARVD 2 antibody
  • ARVD2 antibody
  • Brain ryanodine receptor calcium release channel antibody
  • Brain type ryanodine receptor antibody
  • Cardiac muscle ryanodine receptor antibody
  • Cardiac muscle ryanodine receptor calcium release channel antibody
  • Cardiac muscle type ryanodine receptor antibody
  • CCO antibody
  • Central core disease of muscle antibody
  • HBRR antibody
  • hRYR 2 antibody
  • hRYR2 antibody
  • MHS antibody
  • MHS1 antibody
  • Ryanodine receptor 1 (skeletal) antibody
  • Ryanodine receptor 1 antibody
  • Ryanodine receptor 2 (cardiac) antibody
  • Ryanodine receptor 2 antibody
  • Ryanodine receptor 3 antibody
  • Ryanodine receptor type1 antibody
  • RYDR antibody
  • RYR 1 antibody
  • RYR 2 antibody
  • RYR 3 antibody
  • RYR antibody
  • RYR1 antibody
  • RYR2 antibody
  • RYR3 antibody
  • Sarcoplasmic reticulum calcium release channel antibody
  • Skeletal muscle calcium release channel antibody
  • Skeletal muscle ryanodine receptor antibody
  • Skeletal muscle type ryanodine receptor antibody
  • SKRR antibody
  • Type 1 like ryanodine receptor antibody
  • VTSIP antibody
see all

Anti-Ryanodine Receptor antibody [C3-33] images:

  Immunohistochemistry (Frozen sections) - Ryanodine Receptor antibody [C3-33] (ab2827)

Immunohistochemistry (Frozen sections) - Ryanodine Receptor antibody [C3-33] (ab2827)

ab2827 at 1/100 staining rabbit atrium tissue sections by IHC-Fr. The tissue was paraformaldehyde fixed and blocked with serum prior to incubation with the antibody for 12 hours. An Alexa-Fluor ® 594 conjugated goat anti-mouse IgG antibody was used as the secondary.

This image is courtesy of an anonymous Abreview

See Abreview

  Immunohistochemistry (Frozen sections) - Ryanodine Receptor antibody [C3-33] (ab2827)

Immunohistochemistry (Frozen sections) - Ryanodine Receptor antibody [C3-33] (ab2827)

ab2827 staining Ryanodine Receptor in dog skeletal muscle section by Immunohistochemistry (Frozen sections). Tissue underwent fixation in acetone. The primary antibody was diluted 1/100 and incubated with sample for 12 hours at 4°C. A FITC-conjugated goat polyclonal to mouse IgG1, diluted 1/200 was used as secondary.

This image is courtesy of an anonymous Abreview

See Abreview

References for Anti-Ryanodine Receptor antibody [C3-33] (ab2827)

This product has been referenced in:

  • Briston SJet al. Impaired {beta}-adrenergic responsiveness accentuates dysfunctional excitation-contraction coupling in an ovine model of tachypacing-induced heart failure. J Physiol 589:1367-82 (2011).Read more (PubMed: 21242250) »
  • Jaehnig EJet al. Increased susceptibility to isoproterenol-induced cardiac hypertrophy and impaired weight gain in mice lacking the histidine-rich calcium-binding protein. Mol Cell Biol 26:9315-26 (2006). WB.Read more (PubMed: 17030629) »

See all 10 publications for this product

Publishing research using ab2827? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"