Ryanodine Receptor antibody [C3-33] (ab2827)

Product Name 

Ryanodine Receptor antibody [C3-33]

Product type 

Primary antibodies

Description 

Mouse monoclonal [C3-33] to Ryanodine Receptor

Immunogen 

Canine cardiac ryanodine receptor

Reacts with 
(species key)

Hu, Rat, Amph, Chk, Dog, Fsh, Gpig, Rb

Specificity 

Detects ryanodine receptor 2 (RyR 2) and weakly detects RyR 1.

Tested applications 
(see key)

Flow Cyt, IHC-Fr, IHC-P, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
Immunohistochemistry: use at a concentration of 1 µg/ml.
Immunoprecipitation: Assay dependent.
Western Blot:use at a concentration of 1 µg/ml.
Flow Cyt: Assay dependent.
Not tested in other applications.

Optimal dilutions / concentrations should be determined by the end user.

By Western blot, this antibody detects a 565 kDa protein representing the ryanodine receptor.
In non-mammalian vertebrates, a doublet is seen at 565 kDa representing the alpha and beta isoforms of the receptor.

Immunohistochemical staining of RyR in rat cardiac tissue results in intense staining of the myofiber, which is consistent with sarcoplasmic reticulum localization.

Cellular localization 

Membrane; Multi-pass membrane protein

Research areas 

Cancer >> Cancer Metabolism >> Response to hypoxia
Cardiovascular >> Heart >> Contractility >> Inotropics
Cardiovascular >> Heart >> Cardiac arrhythmias
Neuroscience >> Neurotransmission >> Calcium Signaling >> Calcium Channels >> Ryanodine Receptors

Relevance 

The Ryanodine Receptor (RyR) is the channel responsible for calcium release from muscle cell Sarcoplasmic Reticulum (SR) and also plays a role in calcium regulation in non-muscle cells. The RyR exists as a homotetramer and is predicted to have a short cytoplasmic C-terminus and 4-10 transmembrane domains. The remainder of the protein, termed the "foot" region, is located in the cytoplasm between the transverse tubule and the SR. Mammalian RyR isoforms are the product of three different genes: RyR-1 is expressed predominantly in skeletal muscle and areas of the brain; RyR-2 is expressed predominantly in heart muscle but also found in the stomach, endothelial cells and diffuse areas of the brain; and RyR-3 is found in smooth muscle and the brain (striatum, thalamus and hippocampus). In non-mammalian vertebrates, the RyR isoforms are termed alpha, beta and cardiac which correlate loosely to the mammalian RyR-1, RyR-3 and RyR-2 isoforms respectively.
Defects in the RYR2 gene are the cause of several heart diseases: 1) familial arrhythmogenic right ventricular dysplasia 2 (ARVD2), also known as arrhythmogenic right ventricular cardiomyopathy 2 (ARVC2), 2) an autosomal dominant form of stress-induced polymorphic ventricular tachycardia (VTSIP), also known as catecholaminergic polymorphic ventricular tachycardia (CPVT) and 3) familial polymorphic ventricular tachycardia (FPVT).
Ryanodine Receptor 2 contains several phosphorylatable sites. Specifically, Ser-2030 and Ser-2809 (or at Ser-2808 depending on the species) can be phosphoryated by protein kinase A (PKA) and Ser-2815 (or at Ser-2814 depending on the species) can be phosphorylated by CaMKII (Ca2+/calmodulin-dependent protein kinase II).

The Ryanodine Receptor (RyR) is the channel responsible for the release of calcium from the sarcoplasmic reticulum (SR) in muscle cells and also plays a role in calcium regulation in non-muscle cells. The RyR exists as a homotetramer and is predicted to have a short cytoplasmic C-terminus and 4-10 transmembrane domains; the remainder of the protein, termed the "foot" region is located in the cytoplasm between the T-tubule and the SR. The mammalian RyR is the product of three different genes: RyR 1, which is expressed predominantly in skeletal muscle and areas of the brain, RyR 2, which is expressed predominantly in the heart muscle but also found in the stomach, endothelial cells and diffuse areas of the brain, and RyR-3 which is found in smooth muscle and the brain (striatum, thalamus and hippocampus).

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

C3-33

Isotype 

IgG1

Purity 

Ascites

Storage buffer 

PBS with 0.05% sodium azide

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

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Ryanodine Receptor antibody [C3-33] images:

References for Ryanodine Receptor antibody [C3-33] (ab2827)

  This product has been used in: (two most recent references)

Jaehnig EJ et al. Increased susceptibility to isoproterenol-induced cardiac hypertrophy and impaired weight gain in mice lacking the histidine-rich calcium-binding protein. Mol Cell Biol 26:9315-26 (2006). WB. PubMed: 17030629

Koulen P et al. Differentially distributed IP3 receptors and Ca2+ signaling in rod bipolar cells. Invest Ophthalmol Vis Sci 46:292-8 (2005). WB. PubMed: 15623787

See all 9 publication references for this product.


If you publish research using ab2827 please let us know so that we can cite the reference on this datasheet.

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