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Immunohistochemistry (Frozen sections) - Ryanodine Receptor antibody [C3-33] (ab2827)
![Immunohistochemistry (Frozen sections) - Ryanodine Receptor antibody [C3-33] (ab2827)](/ps/datasheet/images/ab2827_2.jpg)
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Immunohistochemistry (Frozen sections) - Ryanodine Receptor antibody [C3-33] (ab2827)
![Immunohistochemistry (Frozen sections) - Ryanodine Receptor antibody [C3-33] (ab2827)](/ps/datasheet/images/2/ab2827/Ryanodine-Receptor-Primary-antibodies-ab2827-2.jpg)
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Product Name
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Ryanodine Receptor antibody [C3-33]
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See all Ryanodine Receptor antibodies (6)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [C3-33] to Ryanodine Receptor
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Immunogen
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Canine cardiac ryanodine receptor
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Reacts with
(species key)
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Hu, Rat, Amph, Chk, Dog, Fsh, Gpig, Rb
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Specificity
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Detects ryanodine receptor 2 (RyR 2) and weakly detects RyR 1.
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Tested applications
(see key)
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Flow Cyt, IHC-Fr, IHC-P, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions Immunohistochemistry: use at a concentration of 1 µg/ml. Immunoprecipitation: Assay dependent. Western Blot:use at a concentration of 1 µg/ml. Flow Cyt: Assay dependent. Not tested in other applications.
Optimal dilutions / concentrations should be determined by the end user.
By Western blot, this antibody detects a 565 kDa protein representing the ryanodine receptor. In non-mammalian vertebrates, a doublet is seen at 565 kDa representing the alpha and beta isoforms of the receptor.
Immunohistochemical staining of RyR in rat cardiac tissue results in intense staining of the myofiber, which is consistent with sarcoplasmic reticulum localization.
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Cellular localization
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Membrane; Multi-pass membrane protein
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Research areas
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Cancer >> Cancer Metabolism >> Response to hypoxia Cardiovascular >> Heart >> Contractility >> Inotropics Cardiovascular >> Heart >> Cardiac arrhythmias Neuroscience >> Neurotransmission >> Calcium Signaling >> Calcium Channels >> Ryanodine Receptors
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Relevance
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The Ryanodine Receptor (RyR) is the channel responsible for calcium release from muscle cell Sarcoplasmic Reticulum (SR) and also plays a role in calcium regulation in non-muscle cells. The RyR exists as a homotetramer and is predicted to have a short cytoplasmic C-terminus and 4-10 transmembrane domains. The remainder of the protein, termed the "foot" region, is located in the cytoplasm between the transverse tubule and the SR. Mammalian RyR isoforms are the product of three different genes: RyR-1 is expressed predominantly in skeletal muscle and areas of the brain; RyR-2 is expressed predominantly in heart muscle but also found in the stomach, endothelial cells and diffuse areas of the brain; and RyR-3 is found in smooth muscle and the brain (striatum, thalamus and hippocampus). In non-mammalian vertebrates, the RyR isoforms are termed alpha, beta and cardiac which correlate loosely to the mammalian RyR-1, RyR-3 and RyR-2 isoforms respectively. Defects in the RYR2 gene are the cause of several heart diseases: 1) familial arrhythmogenic right ventricular dysplasia 2 (ARVD2), also known as arrhythmogenic right ventricular cardiomyopathy 2 (ARVC2), 2) an autosomal dominant form of stress-induced polymorphic ventricular tachycardia (VTSIP), also known as catecholaminergic polymorphic ventricular tachycardia (CPVT) and 3) familial polymorphic ventricular tachycardia (FPVT). Ryanodine Receptor 2 contains several phosphorylatable sites. Specifically, Ser-2030 and Ser-2809 (or at Ser-2808 depending on the species) can be phosphoryated by protein kinase A (PKA) and Ser-2815 (or at Ser-2814 depending on the species) can be phosphorylated by CaMKII (Ca2+/calmodulin-dependent protein kinase II).
The Ryanodine Receptor (RyR) is the channel responsible for the release of calcium from the sarcoplasmic reticulum (SR) in muscle cells and also plays a role in calcium regulation in non-muscle cells. The RyR exists as a homotetramer and is predicted to have a short cytoplasmic C-terminus and 4-10 transmembrane domains; the remainder of the protein, termed the "foot" region is located in the cytoplasm between the T-tubule and the SR. The mammalian RyR is the product of three different genes: RyR 1, which is expressed predominantly in skeletal muscle and areas of the brain, RyR 2, which is expressed predominantly in the heart muscle but also found in the stomach, endothelial cells and diffuse areas of the brain, and RyR-3 which is found in smooth muscle and the brain (striatum, thalamus and hippocampus).
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Database links
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The links below go to external sites and will open in a new browser window
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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C3-33
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Isotype
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IgG1
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Purity
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Ascites
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Storage buffer
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PBS with 0.05% sodium azide Material safety datasheet (MSDS) for this product: Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Ryanodine Receptor antibody [C3-33] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Ryanodine Receptor antibody [C3-33] images, references, products related to ab2827 and other tools.
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Ryanodine Receptor antibody [C3-33] images:
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Immunohistochemistry (Frozen sections) - Ryanodine Receptor antibody [C3-33] (ab2827)
ab2827 at 1/100 staining rabbit atrium tissue sections by IHC-Fr. The tissue was paraformaldehyde fixed and blocked with serum prior to incubation with the antibody for 12 hours. An Alexa-Fluor ® 594 conjugated goat anti-mouse IgG antibody was used as the secondary. This image is courtesy of an anonymous Abreview
See Abreview
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Immunohistochemistry (Frozen sections) - Ryanodine Receptor antibody [C3-33] (ab2827)
ab2827 staining Ryanodine Receptor in dog skeletal muscle section by Immunohistochemistry (Frozen sections). Tissue underwent fixation in acetone. The primary antibody was diluted 1/100 and incubated with sample for 12 hours at 4°C. A FITC-conjugated goat polyclonal to mouse IgG1, diluted 1/200 was used as secondary. This image is courtesy of an anonymous Abreview
See Abreview
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References for Ryanodine Receptor antibody [C3-33] (ab2827)
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This product has been used in: (two most recent references)
Jaehnig EJ et al. Increased susceptibility to isoproterenol-induced cardiac hypertrophy and impaired weight gain in mice lacking the histidine-rich calcium-binding protein. Mol Cell Biol 26:9315-26 (2006). WB. PubMed: 17030629
Koulen P et al. Differentially distributed IP3 receptors and Ca2+ signaling in rod bipolar cells. Invest Ophthalmol Vis Sci 46:292-8 (2005). WB. PubMed: 15623787
See all 9 publication references for this product.
If you publish research using ab2827 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Ryanodine Receptor
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Ryanodine Receptor antibody [C3-33] - more information
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"