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Anti-Ryanodine Receptor (phospho S2808) antibody (ab59225)

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1 question for ab59225

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Question 1

Tuesday 24-January-2012

Thank you very much for your kind, prompt reply. Do you have other specific phospho antibodies as well?

ANSWER:

  

Thank you for your reply.


We have a number of phospho specific antibodies in our catalog, but ab59225 is the only phospho specific antibody we have to Ryanodine Receptors.


In order to determine specifically which residues are phosphorylated, you can perform an IP with an antibody to total ryanodine receptor (such as ab2868) and analyze the product in a mass spectrometer.


Another possibility would be to perform a sandwich ELISA using an antibody to total ryanodine receptor for capture and an antibody to a phosphorylated residue for detection. While this approach would not tell you which residues specifically are phosphorylated, you would be able to differentiate between phosphoserine (ab6639), phosphothreonine (ab9337), and phosphotyrosine (ab9319).


I hope this helps, please let me know if you need any additional information.

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