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Products:Neuroscience >> Neurotransmission >> Calcium Signaling >> Calcium Channels >> Ryanodine Receptors
Overview
Product name
Anti-Ryanodine Receptor (phospho S2808) antibody
See all Ryanodine Receptor products (7) ...
Description
Rabbit polyclonal to Ryanodine Receptor (phospho S2808)
Specificity
ab59225 detects endogenous levels of Ryanodine Receptor only when phosphorylated at serine 2808.
Tested applications
IHC-P, ELISA, WBmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic phosphopeptide derived from human Ryanodine Receptor around the phosphorylation site of serine 2808 (R-I-SP-Q-T).
Positive control
Human brain tissue.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab59225 was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Response to hypoxia
Cardiovascular >> Heart >> Contractility >> Inotropics
Cardiovascular >> Heart >> Cardiac arrhythmias
Neuroscience >> Neurotransmission >> Calcium Signaling >> Calcium Channels >> Ryanodine Receptors
Applications
Show applications key
Our Abpromise guarantee covers the use of ab59225 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/50 - 1/100.
ELISA
ELISA: 1/5000ELISA: 1/5000
WB: Use at an assay dependent dilution. (PubMed: 21156754)
Target
Relevance
The Ryanodine Receptor (RyR) is the channel responsible for calcium release from muscle cell Sarcoplasmic Reticulum (SR) and also plays a role in calcium regulation in non-muscle cells. The RyR exists as a homotetramer and is predicted to have a short cytoplasmic C-terminus and 4-10 transmembrane domains. The remainder of the protein, termed the "foot" region, is located in the cytoplasm between the transverse tubule and the SR. Mammalian RyR isoforms are the product of three different genes: RyR-1 is expressed predominantly in skeletal muscle and areas of the brain; RyR-2 is expressed predominantly in heart muscle but also found in the stomach, endothelial cells and diffuse areas of the brain; and RyR-3 is found in smooth muscle and the brain (striatum, thalamus and hippocampus). In non-mammalian vertebrates, the RyR isoforms are termed alpha, beta and cardiac which correlate loosely to the mammalian RyR-1, RyR-3 and RyR-2 isoforms respectively. Defects in the RYR2 gene are the cause of several heart diseases: 1) familial arrhythmogenic right ventricular dysplasia 2 (ARVD2), also known as arrhythmogenic right ventricular cardiomyopathy 2 (ARVC2), 2) an autosomal dominant form of stress-induced polymorphic ventricular tachycardia (VTSIP), also known as catecholaminergic polymorphic ventricular tachycardia (CPVT) and 3) familial polymorphic ventricular tachycardia (FPVT). Ryanodine Receptor 2 contains several phosphorylatable sites. Specifically, Ser-2030 and Ser-2809 (or at Ser-2808 depending on the species) can be phosphoryated by protein kinase A (PKA) and Ser-2815 (or at Ser-2814 depending on the species) can be phosphorylated by CaMKII (Ca2+/calmodulin-dependent protein kinase II).
Cellular localization
Membrane; Multi-pass membrane protein
Alternative names
- Arrhythmogenic right ventricular dysplasia 2 antibody
- ARVC 2 antibody
- ARVC2 antibody
see all
Database links
- Entrez Gene: 6261 Human
- Entrez Gene: 6262 Human
- Entrez Gene: 6263 Human
- Entrez Gene: 20190 Mouse
- Entrez Gene: 20191 Mouse
- Entrez Gene: 20192 Mouse
- Omim: 180901 Human
- Omim: 180902 Human
see all
Anti-Ryanodine Receptor (phospho S2808) antibody images:
Immunohistochemistry (Paraffin-embedded sections) - Ryanodine Receptor (phospho S2808) antibody (ab59225)
ab59225 (1/50 dilution) staining Ryanodine Receptor in human brain tissue by Immunohistochemistry (Paraffin section); in the absence or presence of the immunizing peptide.
Western blot - Anti-Ryanodine Receptor (phospho S2808) antibody (ab59225)
Representative Western blot showing Ser(P)2844-RYR1 levels before and after treatment with PP1. The bottom panel summarizes the amount of specific [3H]Ry binding to muscle membranes isolated from WT, Het, and Hom T4826I mice with and without PP1 treatment. PP1 effectively dephosphorylates Ser(P)2844-RYR1 but has no effect on [3H]Ry binding levels, which remain significantly higher in preparations from Het and Hom mutants. ab59225 was used at a 1/2000 dilution.
Image from Barrientos GC et al, J Biol Chem. 2012 Jan 20;287(4):2863-76. Epub 2011 Dec 2, FIg 6. DOI 10.1074/jbc.M111.307926 January 20, 2012 The Journal of Biological Chemistry, 287, 2863-2876.
References for Anti-Ryanodine Receptor (phospho S2808) antibody (ab59225)
This product has been referenced in:
- Barrientos GCet al. Gene Dose Influences Cellular and Calcium Channel Dysregulation in Heterozygous and Homozygous T4826I-RYR1 Malignant Hyperthermia-susceptible Muscle. J Biol Chem 287:2863-76 (2012). WB; Mouse.Read more (PubMed: 22139840) »
- Feng Wet al. Functional and Biochemical Properties of Ryanodine Receptor Type 1 Channels from Heterozygous R163C Malignant Hyperthermia-Susceptible Mice. Mol Pharmacol 79:420-31 (2011). WB; Mouse.Read more (PubMed: 21156754) »
See all 2 publications for this product
Publishing research using ab59225? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"