AbcamBiochemicals

(S)-Propranolol Fluorescent ligand (Red) (ab118169)

Overview

  • Product name(S)-Propranolol Fluorescent ligand (Red)
  • Description

    Fluorescent β2 adrenoceptor antagonist

  • Alternative names
    • β2-633-AN2
  • Biological descriptionFluorescent β2 antagonist (pKD values are 7.76, 9.21 and 7.09 for β1, β2 and β3 respectively). Wide range of applications which include localizing receptor distribution in tissues and cells, and live-cell imaging of receptor kinetics. Able to antagonize the activity of isoprenaline in vitro. No intrinsic agonist activity.

    Excitation wavelength: 633 nm

    Emission wavelength: 650 nm

  • Purity> 97%

Properties

    Applications

    Our Abpromise guarantee covers the use of ab118169 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Small Molecule Fluorescent Optical Detection Use at an assay dependent concentration.
    Receptor Localization Use at an assay dependent concentration.
    Fluorescent Cell Imaging Use at an assay dependent concentration.

    (S)-Propranolol Fluorescent ligand (Red) images

    • Left: ab118169 ligand (30 nM) binding to live CHO cells expressing β2 adrenergic receptors. Right: Binding blocked by the unlabelled competitor ICI118551 (10 µM). Nuclei have been counter-stained with Hoechst.

    References for (S)-Propranolol Fluorescent ligand (Red) (ab118169)

    ab118169 has not yet been referenced specifically in any publications.

    Product Wall

    You are correct that BODIPY is in fact highly lipid soluble. Currently, as we do not carry out our own research for these products, we are unable to know if this compound is able to bind to intracellular receptors. As this range of compounds were d...

    Read More

    These compounds are generally not cell-permeable and have been developed to bind to extracellular receptors. There might be a small amount of receptor internalisation but this is not the primary effect. If you are able to work out a way to transport...

    Read More

    Thank you for contacting us.

    We unfortunately do not sell Bodipy dyes. These however can be easily available form XXXX


    Please click the link below to know which Bodipy dye is attached to products;

    http://www.abcam.com/in...

    Read More

    Thank you for contacting us and for your interest in our products.

    I am sorry to confirm that as far as we are aware,the fluorescent ligandshave not been tested in flow cytometry. All tested and guaranteed applications are specified on our d...

    Read More

    I have received further information from lab about the solubility.

    They said, there are no known problems of using these compounds in aqueous buffer, however no specific numeric data exists. These fluorescent ligands can be used in other organ...

    Read More

    Thank you for contacting us.

    The product can be dissolved to 10 mM or 1 mM (to give a larger volume) in DMSO.

    · Appropriate final DMSO concentration is 0.1% (in buffer)

    · We are not aware of aqueous solubility o...

    Read More

    Thank you for contacting us.

    Although we do not have any in-house comparisons of the fluorescent ligands you asked about and BODIPY-TMR-CGP, I did find a paper that compared alprenolol and propranolol to CGP-12177 for binding efficiency, and...

    Read More

    Thank you for contacting Abcam.

    All of these fluorescent ligands are not suitable for use in paraffin embedded sections as non-specific binding will be seen. The best results are seen using live cells, as this will allow the ligand to bind t...

    Read More

    Thank you for contacting Abcam.

    I have included a pdf file which contains the pKd values for the products as you have asked. This information and more is also available
    on the individual product pages on the abcam website (e.g. http://www...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"