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Products:Signal Transduction >> Metabolism >> Amino Acids
Overview
Product name
Anti-S adenosylhomocysteine hydrolase antibody - Aminoterminal end
See all S adenosylhomocysteine hydrolase products (3) ...
Description
Rabbit polyclonal to S adenosylhomocysteine hydrolase - Aminoterminal end
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Recombinant Fragment
Predicted to work with
Mouse, Rat, Human
Immunogen
Synthetic peptide corresponding to N terminal residues of human Adenosylhomocysteine hydrolase
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 50% Glycerol, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
-
Western blot - S adenosylhomocysteine hydrolase antibody - Aminoterminal end (ab56146)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab56146 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlPredicted molecular weight: 48 kDa.(This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.)
ELISA: Use at an assay dependent dilution.
Target
Function
Adenosylhomocysteine is a competitive inhibitor of S-adenosyl-L-methionine-dependent methyl transferase reactions; therefore adenosylhomocysteinase may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine.
Pathway
Amino-acid biosynthesis; L-homocysteine biosynthesis; L-homocysteine from S-adenosyl-L-homocysteine: step 1/1.
Involvement in disease
Defects in AHCY are the cause of hypermethioninemia with S-adenosylhomocysteine hydrolase deficiency (HMAHCHD) [MIM:613752]. A metabolic disorder characterized by hypermethioninemia associated with failure to thrive, mental and motor retardation, facial dysmorphism with abnormal hair and teeth, and myocardiopathy.
Sequence similarities
Belongs to the adenosylhomocysteinase family.
Cellular localization
Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Target information above from: UniProt accessionP23526
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Adenosylhomocysteinase antibody
- AdoHcyase antibody
- ahcY antibody
see all
Database links
- Entrez Gene: 191 Human
- Entrez Gene: 269378 Mouse
- Entrez Gene: 29443 Rat
- Omim: 180960 Human
- SwissProt: P23526 Human
- SwissProt: P50247 Mouse
- SwissProt: P10760 Rat
- Unigene: 388004 Human
see all
Anti-S adenosylhomocysteine hydrolase antibody - Aminoterminal end images:
Western blot - S adenosylhomocysteine hydrolase antibody - Aminoterminal end (ab56146)
All lanes : Anti-S adenosylhomocysteine hydrolase antibody - Aminoterminal end (ab56146) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size : 48 kDa
Observed band size : 48 kDa
References for Anti-S adenosylhomocysteine hydrolase antibody - Aminoterminal end (ab56146)
ab56146 has not yet been referenced specifically in any publications.
Publishing research using ab56146? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"