All mammalian cells contain low levels of nitrosylated proteins, that are thought to be regulated by Snitrosylation and denitrosylation. S nitrosylation of proteins serves as a ubiquitous post-translational modification that dynamically regulates a broad functional spectrum of proteins. The majority of these proteins are regulated by S nitrosylation on a single critical cysteine residue within an acidic/basic or
hydrophobic structural motif, that may also be subject to oxygen or glutathione dependent modification, suggesting that S nitrosylation is a prototypic redox signal.
Western blot - Anti-S-nitrosocysteine antibody [HY8E12] (ab94930)Image from Ding SY et al., J Biol Chem. 2011 May 13;286(19):16768-74. Epub 2011 Mar 25.; Fig 2.; doi: 10.1074/jbc.M110.192799; May 13, 2011 The Journal of Biological Chemistry, 286, 16768-16774.
Image from Ding SY et al., J Biol Chem. 2011 May 13;286(19):16768-74. Epub 2011 Mar 25.; Fig 2.; doi: 10.1074/jbc.M110.192799; May 13, 2011 The Journal of Biological Chemistry, 286, 16768-16774.
Mouse pancreatic islets were treated with 30 nm GLP-1 for 10 min prior to lysis. GCK was immunoprecipitated and assessed for S-nitrosocysteine by Western blotting using ab94930.
Bahnson ES et al. Targeted Nitric Oxide Delivery by Supramolecular Nanofibers for the Prevention of Restenosis After Arterial Injury. Antioxid Redox Signal24:401-18 (2016).
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