Chemical/ Small Molecule corresponding to S-nitrosocysteine conjugated to Bovine Serum Albumin (BSA).
Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Constituents: 50% Glycerol
Concentration information loading...
Ammonium Sulphate Precipitation
Ascites production was performed in BALB/c mice. The ascitic fluid was purified by ammonium sulfate precipitation.
All mammalian cells contain low levels of nitrosylated proteins, that are thought to be regulated by Snitrosylation and denitrosylation. S nitrosylation of proteins serves as a ubiquitous post-translational modification that dynamically regulates a broad functional spectrum of proteins. The majority of these proteins are regulated by S nitrosylation on a single critical cysteine residue within an acidic/basic or
hydrophobic structural motif, that may also be subject to oxygen or glutathione dependent modification, suggesting that S nitrosylation is a prototypic redox signal.
Western blot - Anti-S-nitrosocysteine antibody [HY8E12] (ab94930)Image from Ding SY et al., J Biol Chem. 2011 May 13;286(19):16768-74. Epub 2011 Mar 25.; Fig 2.; doi: 10.1074/jbc.M110.192799; May 13, 2011 The Journal of Biological Chemistry, 286, 16768-16774.
Image from Ding SY et al., J Biol Chem. 2011 May 13;286(19):16768-74. Epub 2011 Mar 25.; Fig 2.; doi: 10.1074/jbc.M110.192799; May 13, 2011 The Journal of Biological Chemistry, 286, 16768-16774.
Mouse pancreatic islets were treated with 30 nm GLP-1 for 10 min prior to lysis. GCK was immunoprecipitated and assessed for S-nitrosocysteine by Western blotting using ab94930.
Bahnson ES et al. Targeted Nitric Oxide Delivery by Supramolecular Nanofibers for the Prevention of Restenosis After Arterial Injury. Antioxid Redox Signal24:401-18 (2016).
Read more (PubMed: 26593400) »