Products:Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calcium Binding Proteins
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I am interested in using this antibody for IHC. Your information says that it has not been used for this before. Does this mean that it does not work? If i buy this antibody and it does not work in this application how do you deal with that? What experience do people have in testing unknown antibodies in IHC. |
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ANSWER: |
Thank you for your enquiry and your interest in our products. This antibody has not yet been tested for use of IHC. It does not necessary mean that it is not suitable for this application, it has just not been characterised yet. Should you decide to go ahead and test this antibody, please let us know how you get on, and in return we will forward a USD15/EUR15/GBP10 Amazon gift voucher. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Anti-S100 alpha 2 antibody [SH-L1] (ab11180) at 1/500 dilution + Human pancreas - whole cell lysate at 20 µg
Secondary
HRP conjugated goat anti-mouse antibody at 1/2000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 11 kDa
Observed band size : 10 kDa (why is the actual band size different from the predicted?)
Additional bands at : 20 kDa (possible non-specific binding).
Exposure time : 1 minute
This image is courtesy of an anonymous Abreview
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