You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Reassurance, Refunds & Replacements

If your product does not perform as described on this datasheet, we will refund or replace your product...

Read our guarantee »
 

Anti-S100 alpha 2 antibody [SH-L1] (ab11180)

CodeSizePriceAbpointsAvailability
    
Add to basket
 
  • -
  •   
  •   
  •   
  •  
  •   
  •   
Updating...

Overview

Product name

Anti-S100 alpha 2 antibody [SH-L1]
See all S100 alpha 2 products (5) ...

Description

Mouse monoclonal [SH-L1] to S100 alpha 2

Specificity

The product does not react with other members of the EF-hand family including calmodulin, parvalbumin, intestinal calcium-binding protein, S100A6 (calcyclin), caltropin, the alpha chain of S100 (i.e. in S100a and S100ao), or the beta chain (i.e. in S100a and S100b).

Tested applications

ELISA, WB, IP, IHC-Fr, ICC/IFmore details

Cross reactivity

Reacts with

Rat, Goat, Cow, Cat, Dog, Human, Pig, Lizard

Immunogen

Full length native protein (purified) (Pig).

Epitope

Monoclonal Anti S100A2 (S100L) recognizes an epitope located on S100A2 (formerly known as S100L).

Positive control

MDBK cells (immunofluorescence).

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 15mM Sodium Azide

Concentration

Concentration information loading...

Purity

Ascites

Clonality

Monoclonal

Clone number

SH-L1

Isotype

IgG1

Research areas

Cell Biology >> Cell Cycle >> Cell differentiation
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calcium Binding Proteins

  • Western blot - S100 alpha 2 antibody [SH-L1] (ab11180)Western blot - S100 alpha 2 antibody [SH-L1] (ab11180) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab11180 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

May act as a modulator against excess calcium accumulation in normal human mammary epithelial cells. May also play a role in suppressing tumor cell growth.

Tissue specificity

A subset of epithelial cells including normal human mammary epithelial cells and keratinocytes.

Sequence similarities

Belongs to the S-100 family.
Contains 2 EF-hand domains.

Developmental stage

Preferentially expressed in normal human mammary epithelial cells as opposed to tumor-derived ones. The level of S100L was shown to correlate inversely with tumor progression.

Target information above from: UniProt accessionP29034 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • S100 calcium binding protein A2 antibody
  • CAN19 antibody
  • MGC111539 antibody
  • Protein S 100L antibody
  • Protein S-100L antibody
  • Protein S100 A2 antibody
  • Protein S100-A2 antibody
  • Protein S100L antibody
  • S100 A2 antibody
  • S100 calcium-binding protein A2 antibody
  • S100A2 antibody
  • S100L antibody
  • S10A2_HUMAN antibody
see all

Database links

Anti-S100 alpha 2 antibody [SH-L1] images:

  Western blot - S100 alpha 2 antibody [SH-L1] (ab11180)

Western blot - S100 alpha 2 antibody [SH-L1] (ab11180)

Anti-S100 alpha 2 antibody [SH-L1] (ab11180) at 1/500 dilution + Human pancreas - whole cell lysate at 20 µg

Secondary
HRP conjugated goat anti-mouse antibody at 1/2000 dilution
developed using the ECL technique

Performed under reducing conditions.

Predicted band size : 11 kDa
Observed band size : 10 kDa (why is the actual band size different from the predicted?)
Additional bands at : 20 kDa (possible non-specific binding).

Exposure time : 1 minute

This image is courtesy of an anonymous Abreview

See Abreview

References for Anti-S100 alpha 2 antibody [SH-L1] (ab11180)

This product has been referenced in:

  • Zech VFet al. Prognostic and diagnostic relevance of hnRNP A2/B1, hnRNP B1 and S100 A2 in non-small cell lung cancer. Cancer Detect Prev 30:395-402 (2006). IHC-Fr; Human.Read more (PubMed: 17067748) »
  • Stradal TBet al. Mapping the zinc ligands of S100A2 by site-directed mutagenesis. J Biol Chem 275:13219-27 (2000). WB; Human.Read more (PubMed: 10788426) »

See all 2 publications for this product

Publishing research using ab11180? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"