S100 alpha 2 antibody [SH-L1] (ab11180)

Product Name 

S100 alpha 2 antibody [SH-L1]

Product type 

Primary antibodies

Description 

Mouse monoclonal [SH-L1] to S100 alpha 2

Immunogen 

Full length native protein (purified) (Pig).

Reacts with 
(species key)

Hu, Rat, Cat, Cow, Dog, Goat, Lzd, Pig

Specificity 

The product does not react with other members of the EF-hand family including calmodulin, parvalbumin, intestinal calcium-binding protein, S100A6 (calcyclin), caltropin, the alpha chain of S100 (i.e. in S100a and S100ao), or the beta chain (i.e. in S100a and S100b).

Epitope 

Monoclonal Anti S100A2 (S100L) recognizes an epitope located on S100A2 (formerly known as S100L).

Tested applications 
(see key)

ELISA, ICC/IF, IHC-Fr, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
ELISA: Use at an assay dependent dilution.
ICC/IF: 1/1000.
IHC-Fr: 1/200. Fix with acetone see (see PMID17067748).
IP: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 11 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

MDBK cells (immunofluorescence).

Research areas 

Cell Biology >> Cell Cycle >> Cell differentiation
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calcium Binding Proteins

Relevance 

S100 alpha 2 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may have a tumor suppressor function. Chromosomal rearrangements and altered expression of this gene have been implicated in breast cancer.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

SH-L1

Isotype 

IgG1

Purity 

Ascites

Storage buffer 

Preservative: 15mM Sodium Azide

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

7.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

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References for S100 alpha 2 antibody [SH-L1] (ab11180)

  This product has been used in: (two most recent references)

Zech VF et al. Prognostic and diagnostic relevance of hnRNP A2/B1, hnRNP B1 and S100 A2 in non-small cell lung cancer. Cancer Detect Prev 30:395-402 (2006). IHC-Fr; Human. PubMed: 17067748

Stradal TB et al. Mapping the zinc ligands of S100A2 by site-directed mutagenesis. J Biol Chem 275:13219-27 (2000). WB; Human. PubMed: 10788426
If you publish research using ab11180 please let us know so that we can cite the reference on this datasheet.

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