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Western blot - S100 alpha 2 antibody (ab89503)
Anti-S100 alpha 2 antibody (ab89503) at 1/500 dilution + Tagged recombinant Human S100 alpha 2 protein at 0.2 µg
Predicted band size : 11 kDa
Observed band size : 37 kDa (why is the actual band size different from the predicted?)
Western blot against tagged recombinant protein immunogen. Predicted band size of immunogen is 37 kDa.
Western blot - S100 alpha 2 antibody (ab89503)
Anti-S100 alpha 2 antibody (ab89503) at 1/500 dilution + A431 cell lysate at 50 µg
Predicted band size : 11 kDa
Observed band size : 10 kDa (why is the actual band size different from the predicted?)
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