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Western blot - S100 alpha 2 antibody (ab89503)
Western blot - S100 alpha 2 antibody (ab89503)
(enlarge)


Western blot - S100 alpha 2 antibody (ab89503)
Western blot - S100 alpha 2 antibody (ab89503)
(enlarge)


Product Name 

S100 alpha 2 antibody

 

See all S100 alpha 2 antibodies (2)...

Product type 

Primary antibodies

Description 

Mouse monoclonal to S100 alpha 2

Immunogen 

Recombinant full length Human S100 alpha 2 (AAH02829) with a proprietary tag.

Reacts with 
(species key)

Hu

Tested applications 
(see key)

ELISA, WB


Abreviews 

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(see key)


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Application notes 
(see key)

Recommended dilutions
ELISA: Use at an assay dependent dilution.
WB: 1/500 - 1/1000. Predicted molecular weight: 11 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

A431 cell lysate. Tagged recombinant Human S100 alpha 2 protein.

Research areas 

Cell Biology >> Cell Cycle >> Cell differentiation
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calcium Binding Proteins

Relevance 

S100 alpha 2 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may have a tumor suppressor function. Chromosomal rearrangements and altered expression of this gene have been implicated in breast cancer.

 

S100 alpha 2 antibody (ab89503)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

6273    (Human)

Omim

    

176993    (Human)

SwissProt

    

P29034    (Human)

Unigene

    

516484    (Human)

Raised in 

Mouse

Clonality 

Monoclonal

Isotype 

IgG2b

Light chain type 

kappa

Purity 

Ascites

Storage buffer 

Preservative: None
Constituents: Ascites

Form 

Liquid

Concentration 

3.299 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this S100 alpha 2 antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for S100 alpha 2 antibody images, references, products related to ab89503 and other tools.

 

S100 alpha 2 antibody images:

Product image  Western blot - S100 alpha 2 antibody (ab89503)

Western blot - S100 alpha 2 antibody (ab89503)

S100 alpha 2 antibody (ab89503) at 1/500 dilution + Tagged recombinant Human S100 alpha 2 protein at 0.2 µg

Predicted band size : 11 kDa
Observed band size : 37 kDa (why is the actual band size different from the predicted?)


Western blot against tagged recombinant protein immunogen. Predicted band size of immunogen is 37 kDa.

 

Product image  Western blot - S100 alpha 2 antibody (ab89503)

Western blot - S100 alpha 2 antibody (ab89503)

S100 alpha 2 antibody (ab89503) at 1/500 dilution + A431 cell lysate at 50 µg

Predicted band size : 11 kDa
Observed band size : 10 kDa (why is the actual band size different from the predicted?)

 
 

Search PubMed (MEDLINE) for references to S100 alpha 2

 

S100 alpha 2 antibody - more information

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S100 alpha 2 antibody - related products:

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