Anti-S100 antibody [4C4.9] - Astrocyte Marker (ab4066)

Thank you for sending the replacement antibody. This one worked very well both in vitro using human induced pluripotent stem cells and in vivo using mouse spinal cord sections.

ANSWER:

That's great news! Thanks so much for letting us know.

Please feel free to contact me if you need anything further.

I have used this antibody on astrocytes grown in culture in ICC and mousespinal cord via IHC-Fr. In neither application did I see any signal using 1:200 dilution of the antibody; using another antibody to an astrocyte marker protein, GFAP, is was able to detect astrocytes in both applications using the same samples.

ANSWER:

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number 1080427.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Thanks for the antibody. It works fine the immunostaining. But I still can not get any signal in western blot, although it was reported that it should work in western blot.



Do you have any other recommendations for western blot?

ANSWER:

Thank you for your reply. After reviewing the customer feedback for ab4066, it appears that the customers who had obtained good WB results were using BSA as a blocking agent. The feedback we had from a customer using 5% milk was that their WB was unsuccessful. It is possible that this antibody may be sensitive to milk and may produce better results when using BSA.

For both ab4066 andab868, you could also try increasing the primary antibody concentration to the range 1:200-1:500 or loading more protein (30-50ug per lane). I hope this helps, please let me know if you need any further assistance.

I am trying to do Western Blot and ICC for s100, a marker for astrocytes. The antibody which I got from Abcam is ab4066. I did not get any signal for WB and very weak signal for ICC. So I wonder what is the problem. If I still can not get any signal after trouble shooting, which antibodies do you recommend for WB and ICC, respectively?

ANSWER:

Thank you for contacting us. I am sorry that ab4066 was not producing satisfactory signal in ICC and WB. Our antibody ab868 is rated highly for use in ICC and WB on human, mouse, rat, cow, and pig samples. If you could please provide some additional details about the protocol and samples you are using, I would be happy to assist you further. If ab4066 is not working as stated on the datasheet and you have purchased it in the past six months, I would be happy to send you ab868 as a free of charge replacement. Could you please provide the following information? 1) What is your original order or PO number? 2) What species and sample type were you testing? How were the cells / tissuefixed or lysed? 3) What permeabilization and/ or blocking agents were used? 4) Could you please tell me the dilution and incubation times for the primary and secondary antibodies? 5) It would be very helpful if you could please provide an image of your WB or ICC.

Wie ist die Konzentration?

ANSWER:

Vielen Dank für Ihren Anruf.

Die Konzentration des S100-Antikörpers ab4066 (Lotnummer 1133341/GR51861-1) beträgt 33,3 µg/ml.

Ich hoffe, diese Information hilft Ihnen weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.