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ab8330 has been referenced in 4 publications.
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Interaction of several monoclonal antibodies with purified S100 protein (1 µg) from human brain in WB, after native gel electrophoresis using the Ornstein-Davis system. Lane 1 = ab14849, Lane 2 = ab8330, ab Lane 3 = ab10203, Lane 4 = ab8334. The doublet band appears at about 20-21 kD,
S-100 calibration curve, from one step assay in streptavidin coated plates. Ab8330 [200 ng/well] was used as capture and Eu- labelled ab10203 [200 ng/well] as detection. Antigen used was S-100 protein from the human brain. Incubated 20 minutes at 20ºC.
ab8330 at a dilution of 1/1000, staining S100 (Alexa 488 secondary at 1/2000) on rat brain tissue (30
NB: No labeling observed following omission of primary antibody.
Sections were viewed using an Axioplan 2 Imaging microscope (Imaging Associates) fitted with 10x, 20x and 40x Plan-Neofluorobjectives (Zeiss, Germany) and images were taken using a AxioCam Hrm digital camera (Zeiss, Germany) and AxioVision software (Imaging Associates).
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