![Immunohistochemistry (PFA perfusion fixed frozen sections) - S100 antibody [8B10] - Astrocyte Marker (ab8330) image](#ab8330_11.jpg)
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Products:Neuroscience >> Neurotransmission >> Calcium Signaling >> Calcium Binding Proteins
Overview
Product name
Anti-S100 antibody [8B10] - Astrocyte Marker
See all S100 products (24) ...
Description
Mouse monoclonal [8B10] to S100 - Astrocyte Marker
Specificity
Specific to S-100 beta beta and alpha beta. It does not recognize the alpha-alpha form or alpha subunit only.
Tested applications
ELISA, IHC - Wholemount, IF, WB, Sandwich ELISAmore details
Cross reactivity
Reacts with
Rat, Human
Immunogen
Full length protein (Human).
Positive control
Human brain.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
PBS with 0.1% sodium azide, pH 7.4
Concentration
Concentration information loading...
Purity
Protein A purified
Purification notes
This antibody is affinity purified.
Clonality
Monoclonal
Clone number
8B10
Myeloma
Sp2/0
Isotype
IgG1
Light chain type
unknown
Research areas
Tags & Cell Markers >> Cell Type Markers >> Neuroscience Markers >> Glial
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Intermediate Filaments >> Class IV >> Neurofilaments
Neuroscience >> Cell Type Marker >> Glia marker >> Astrocyte marker
Neuroscience >> Neurotransmission >> Calcium Signaling >> Calcium Binding Proteins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab8330 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at an assay dependent dilution.
IHC - Wmt: Use at an assay dependent concentration. (PubMed: 21856924)
IF
IF: 1/1000IF: 1/1000
WB: Use at an assay dependent dilution. Predicted molecular weight: 21 kDa.
sELISA: Use at an assay dependent dilution. Can be paired for Sandwich ELISA with Mouse monoclonal [6G1] to S100 (HRP) (ab10203). (Sensitive to EDTA or other bivalent-binding agents. Better performance can be obtained in presence of 5mM CaCL2 in these assay buffers. Can be used as capture antibody in conjunction with ab10203.)
Target
Function
Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites.
Tissue specificity
Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain.
Sequence similarities
Belongs to the S-100 family.
Contains 2 EF-hand domains.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionP23297
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Bpb antibody
- NEF antibody
- Protein S100-A1 antibody
see all
Database links
- Entrez Gene: 6271 Human
- Entrez Gene: 295214 Rat
- Omim: 176990 Human
- SwissProt: P04271 Human
- SwissProt: P23297 Human
- SwissProt: P04631 Rat
- SwissProt: P35467 Rat
- Unigene: 515715 Human
see all
Anti-S100 antibody [8B10] - Astrocyte Marker images:
Western blot
Interaction of several monoclonal antibodies with purified S100 protein (1 µg) from human brain in WB, after native gel electrophoresis using the Ornstein-Davis system. Lane 1 = ab14849, Lane 2 = ab8330, ab Lane 3 = ab10203, Lane 4 = ab8334. The doublet band appears at about 20-21 kD,
ELISA
S-100 calibration curve, from one step assay in streptavidin coated plates. Ab8330 [200 ng/well] was used as capture and Eu- labelled ab10203 [200 ng/well] as detection. Antigen used was S-100 protein from the human brain. Incubated 20 minutes at 20°C.
Immunohistochemistry (PFA perfusion fixed frozen sections) - S100 antibody [8B10] - Astrocyte Marker (ab8330)
![Immunohistochemistry (PFA perfusion fixed frozen sections) - S100 antibody [8B10] - Astrocyte Marker (ab8330)](/ps/datasheet/Images/8/ab8330/ab8330_11.jpg)
ab8330 at a dilution of 1/1000, staining S100 (Alexa 488 secondary at 1/2000) on rat brain tissue (30
NB: No labeling observed following omission of primary antibody.
Sections were viewed using an Axioplan 2 Imaging microscope (Imaging Associates) fitted with 10x, 20x and 40x Plan-Neofluorobjectives (Zeiss, Germany) and images were taken using a AxioCam Hrm digital camera (Zeiss, Germany) and AxioVision software (Imaging Associates).
Protocols
References for Anti-S100 antibody [8B10] - Astrocyte Marker (ab8330)
This product has been referenced in:
- Dai M & Shi X Fibro-vascular coupling in the control of cochlear blood flow. PLoS One 6:e20652 (2011). IHC; Guinea pig.Read more (PubMed: 21673815) »
- Callizot Net al. A new long term in vitro model of myelination. Exp Cell Res : (2011). ICC/IF; Rat.Read more (PubMed: 21777582) »
See all 4 publications for this product
Publishing research using ab8330? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"