Anti-S100 antibody - Astrocyte Marker (ab868)

Thank you for sending the replacement antibody. This one worked very well both in vitro using human induced pluripotent stem cells and in vivo using mouse spinal cord sections.

ANSWER:

That's great news! Thanks so much for letting us know.

Please feel free to contact me if you need anything further.

I have used this antibody on astrocytes grown in culture in ICC and mousespinal cord via IHC-Fr. In neither application did I see any signal using 1:200 dilution of the antibody; using another antibody to an astrocyte marker protein, GFAP, is was able to detect astrocytes in both applications using the same samples.

ANSWER:

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number 1080427.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Thanks for the antibody. It works fine the immunostaining. But I still can not get any signal in western blot, although it was reported that it should work in western blot.



Do you have any other recommendations for western blot?

ANSWER:

Thank you for your reply. After reviewing the customer feedback for ab4066, it appears that the customers who had obtained good WB results were using BSA as a blocking agent. The feedback we had from a customer using 5% milk was that their WB was unsuccessful. It is possible that this antibody may be sensitive to milk and may produce better results when using BSA.

For both ab4066 andab868, you could also try increasing the primary antibody concentration to the range 1:200-1:500 or loading more protein (30-50ug per lane). I hope this helps, please let me know if you need any further assistance.

One of our customers works with Tilapia which many of it's genes share high resembles to Zebra fish. The customer would like to get your opinion regarding antibodies suitability and possibly to order some of them as part of the abreview promotion. It is rather difficult to obtain Tilapia tested antibodies but customer submitted reviews on this species might also help understand their reactivity in Zebra fish samples.   The customer wishes to know about chances for success and your willingness to approve abreview promotions for the following antibodies:   Ab85367 Ab88074 Ab868 Ab14688  

ANSWER:

Thank you for contacting us.  Unfortunately, I was unable to locate any amino acid sequences for Tilapia S100, S100 beta, or Dopamine D2 Receptor with which to compare the immunogen sequences for these antibodies.  For this reason, we have no information about whether or not these antibodies might react with Tilapia.  If the customer would still wish to test them, please let me know and I will be happy to set up testing discount codes. 

No specific signal in pig skin.

ANSWER:

Thank you for your call today and for letting us know about the trouble with ab868.

As we discussed, I have issued credit note # ***. Please pass this credit note number on to your purchasing department. If you have any questions about this credit, you can contact us.credits@abcam.com.

At this time I am not sure why pig is listed as a tested species on our datasheet, but if I can not find this information then I will remove pig from the datasheet.

Please let me know if you have any questions or need anything else and I will be happy to help.